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Molecular characterization of Opisthorchis noverca (Digenea: Opisthorchiidae) based on nuclear ribosomal ITS2 and mitochondrial COI genes

Published online by Cambridge University Press:  15 October 2015

R. Sahu ,
D.K. Biswal ,
B. Roy  and
V. Tandon
R. Sahu
Affiliation:
Department of Zoology, North-Eastern Hill University, Shillong-793022, Meghalaya, India
D.K. Biswal
Affiliation:
Department of Zoology, North-Eastern Hill University, Shillong-793022, Meghalaya, India
B. Roy
Affiliation:
Department of Zoology, North-Eastern Hill University, Shillong-793022, Meghalaya, India
V. Tandon*
Affiliation:
Department of Zoology, North-Eastern Hill University, Shillong-793022, Meghalaya, India
*
*E-mail: tandonveena@gmail.com
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Abstract

Opisthorchiasis is a public health problem in South-East Asian countries and Eastern Europe. The infection implicates mainly two species of Opisthorchis, namely O. viverrini and O. felineus, that occur mostly in fish-eating mammals and humans, although there are rare reports of human cases involving two other species, O. noverca and O. guayaquilensis. Opisthorchis noverca has been reported frequently in dogs and pigs from the Indian subcontinent, with rare reports from cattle and human subjects. With a view to supplementing morphology-based identification of this species, the present study aimed to provide molecular characterization of O. noverca, using rDNA internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome oxidase I (mt COI) markers so as to determine its genetic correlation with other species of Opisthorchiidae, and also to generate a taxon-specific molecular marker based on the ITS2 region. The phylogenetic relationship between O. noverca and other species of the genus was determined using molecular sequence data. To strengthen the result, secondary structure sequence analyses of ITS2 with hemi-compensatory base changes (hCBCs), and amino acid sequence analyses, were also evaluated. Our results confirm that O. noverca is a distinct and valid species.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 5 , September 2016 , pp. 607 - 614
Copyright
Copyright © Cambridge University Press 2015 

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