Hostname: page-component-848d4c4894-2pzkn Total loading time: 0 Render date: 2024-05-14T11:53:07.484Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular identification of Anisakis species from Pleuronectiformes off the Portuguese coast

Published online by Cambridge University Press:  12 April 2024

J.F. Marques ,
H.N. Cabral ,
M. Busi  and
S. D'Amelio
J.F. Marques
Affiliation:
Universidade de Lisboa, Faculdade de Ciências, Instituto de Oceanografia, Campo Grande, 1749-016 Lisboa, Portugal.
H.N. Cabral*
Affiliation:
Universidade de Lisboa, Faculdade de Ciências, Instituto de Oceanografia, Campo Grande, 1749-016 Lisboa, Portugal.
M. Busi
Affiliation:
University of Rome ‘La Sapienza’, Parasitology Section, Department of Sciences of Public Health, Piazzale Aldo Moro 5, 00185 Rome, Italy.
S. D'Amelio
Affiliation:
University of Rome ‘La Sapienza’, Parasitology Section, Department of Sciences of Public Health, Piazzale Aldo Moro 5, 00185 Rome, Italy.
*
* Fax: +351 217 500 207 E-mail: hncabral@fc.ul.pt
Get access Rights & Permissions [Opens in a new window]

Abstract

Anisakid nematodes belonging to the Anisakis simplex complex are highly prevalent in several fish species off the coast of Portugal and are an important zoonotic problem in the Iberian Peninsula. Two reproductively isolated sibling species of the Anisakis simplex complex were identified from Pleuronectiformes inhabiting the Portuguese coast using restriction fragment length polymorphism (RFLP). Recombinant genotypes corresponding to presumptive Anisakis simplex sensu stricto and Anisakis pegreffii hybrids were also detected by this technique, as well as the species Anisakis typica. Although 25 species of Pleuronectiformes were investigated, Anisakis spp. larvae were only found in seven: Arnoglossus imperialis, Arnoglossus laterna, Lepidorhombus boscii, Citharus linguatula, Platichthys flesus, Dicologlossa cuneata and Solea senegalensis. The occurrence of hybrids in relatively sedentary fishes such as the Pleuronectiformes suggests that the Portuguese coast may constitute an area of hybridization and, therefore, is of particular interest for the study of the process of hybridization and speciation for these anisakids.

Type
Research Article
Information
Journal of Helminthology , Volume 80 , Issue 1 , March 2006 , pp. 47 - 51
Copyright
Copyright © Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abollo, E., D'Amelio, S. & Pascual, S. (2001) Fitness of the marine parasitic nematode Anisakis simplex, s.str. in temperate waters of the NE Atlantic. Diseases of Aquatic Organisms 45, 131–139.CrossRefGoogle ScholarPubMed
Abollo, E., Paggi, L., Pascual, S. & D'Amelio, S. (2003) Occurrence of recombinant genotypes of Anisakis simplex, s.s. and Anisakis pegreffii (Nematoda: Anisakidae) in an area of sympatry. Infection, Genetics and Evolution 3, 175–181.CrossRefGoogle Scholar
Audicana, M.T., Ansotegui, I.J., Fernández de Corres, L. & Kennedy, M.W. (2002) Anisakis simplex: dangerous dead and alive? Trends in Parasitology 18, 20–25.CrossRefGoogle ScholarPubMed
Berendzen, P.B. & Dimmick, W.W. (2002) Phylogenetic relationships of Pleuronectiformes based on molecular evidence. Copeia 3, 642–652.CrossRefGoogle Scholar
Berland, B. (1961) Nematodes from some Norwegian marine fishes. Sarsia 2, 1–50.CrossRefGoogle Scholar
Briggs, J.C. (1974) Marine zoogeography, London, McGraw-Hill.Google Scholar
D'Amelio, S., Mathiopoulos, K.D., Santos, C.P., Pugachev, O.N., Webb, S.C., Picanço, M. & Paggi, L. (2000) Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Asacaridoidea) defined by polymerase chain reaction-based restriction fragment length polymorphism. International Journal for Parasitology 30, 223–226.CrossRefGoogle ScholarPubMed
de Corres, L.F., Del Pozo, M.D. & Aizpuru, F. (2001) Prevalence of sensitization to Anisakis simplex in three Spanish areas, regarding the rates of fish consumption. Relevance of Anisakis simplex allergy. Alergologia Immunologia Clinica 16, 337–346.Google Scholar
Ekman, S. (1953) Zoogeography of the sea. London, Sidgwick & Jackson.Google Scholar
Eurostat (2001). http://epp.eurostat.cec.int Google Scholar
Kuris, A.M., Blaustein, A.R. & Alió, J.J. (1980) Hosts as islands. American Naturalist 116, 570–586.CrossRefGoogle Scholar
MacArthur, R.H. & Wilson, E.O. (1967) The theory of island biogeography. Monographs in Population Biology, No. 1, Princeton, Princeton University Press.Google Scholar
Mattiucci, S., Nascetti, G., Cianchi, R., Paggi, L., Arduino, P., Margolis, L., Brattey, J., Webb, S., D'Amelio, S., Orecchia, P. & Bullini, L. (1997) Genetic and ecological data on the Anisakis simplex complex, with evidence for a new species (Nematoda, Ascaridoidea, Anisakidae). Journal of Parasitology 83, 401–416.CrossRefGoogle ScholarPubMed
Mattiucci, S., Paggi, L., Nascetti, G., Santos, S.P., Costa, G., Di Beneditto, A.P., Ramos, R., Argyrou, M., Cianchi, R. & Bullini, L. (2002) Genetic markers in the study of Anisakis typica (Diesing, 1860): larval identification and genetic relationships with other species of Anisakis Dujardin, 1845 (Nematoda: Anisakidae). Systematic Parasitology 51, 159–170.CrossRefGoogle Scholar
Mattiucci, S., Abaunza, P., Ramadori, L. & Nascetti, G. (2004) Genetic identification of Anisakis larvae in European hake from Atlantic and Mediterranean waters for stock recognition. Journal of Fish Biology 65, 495–510.CrossRefGoogle Scholar
Nascetti, G., Paggi, L., Orecchia, P., Mattiucci, S. & Bullini, L. (1983) Two sibling species within Anisakis simplex (Ascaridida: Anisakidae). Parassitologia 25, 239–241.Google Scholar
Nascetti, G., Paggi, L., Orecchia, P., Smith, J., Mattiucci, S. & Bullini, L. (1986) Electrophoretic studies on the Anisakis simplex complex (Ascaridida: Anisakidae) from the Mediterranean and North-east Atlantic. International Journal for Parasitology 16, 633–640.CrossRefGoogle ScholarPubMed
Rosales, M.J., Mascaró, C., Fernandez, C., Luque, F., Moreno, M.S., Parras, L., Cosano, A. & Muñoz, J.R. (1999) Acute intestinal anisakiasis in Spain: a fourth-stage Anisakis simplex larva. Memórias do Instituto Oswaldo Cruz 94, 823–826.CrossRefGoogle Scholar
Svetovidov, A.N. (1984) Gadidae. pp. 680–710 in Whitehead, P.J.P., Bauchot, M.L., Hureau, J.C., Nielsen, J. & Tortonese, E. (Eds) Fishes of the North-eastern Atlantic and Mediterranean. Paris, UNESCO.Google Scholar
Zhu, X.Q., Gasser, R.B., Podolska, M. & Chilton, N.B. (1999) Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. International Journal for Parasitology 28, 1911–1921.CrossRefGoogle Scholar
Zhu, X.Q., D'Amelio, S., Paggi, L. & Gasser, R.B. (2000) Assessing sequence variation in the internal transcribed spacers of ribosomal DNA within and among members of the Contracaecum osculatum complex (Nematoda: Ascaridoidea: Anisakidae). Parasitology Research 86, 677–683.CrossRefGoogle ScholarPubMed