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The prophylactic and therapeutic impact of Trichinella spiralis larvae excretory secretory antigens- loaded Ca-BTC metal organic frameworks on induced murine colitis

Published online by Cambridge University Press:  24 May 2024

E.M. Fawzy ,
M.A. Selim ,
N. E. Mostafa ,
R.M. Abdelhameed ,
A.M. Darwish ,
A.M. Yousef ,
M.A. Alabiad ,
M.N. Ibrahim ,
H.M. Fawzy  and
E.F. Abdel Hamed Open the ORCID record for E.F. Abdel Hamed [Opens in a new window]
E.M. Fawzy
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt
M.A. Selim
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt
N. E. Mostafa
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt
R.M. Abdelhameed
Affiliation:
Department of Applied Organic Chemistry, National Research Centre, Dokki, Giza, Egypt
A.M. Darwish
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt
A.M. Yousef
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt
M.A. Alabiad
Affiliation:
Department of Pathology, Faculty of Medicine, Zagazig University, Egypt
M.N. Ibrahim
Affiliation:
Department of Clinical Laboratories, College of applied Medical Sciences, Jouf University, Qurrayat 77451, KSA
H.M. Fawzy
Affiliation:
Department of Community, Faculty of Medicine, Zagazig University, Sharkia, Egypt
E.F. Abdel Hamed*
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt
*
Corresponding author: E.F. Abdel Hamed; Email: drenasfakhry@gmail.com
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Abstract

Background: Inflammatory bowel disease is an autoimmune disease that affects the gut. T. spiralis larvae (E/S Ags) loaded on calcium-benzene-1,3,5-tricarboxylate metal-organic frameworks (Ca-BTC MOFs) were tested to determine whether they might prevent or cure acetic acid-induced murine colitis. Methods: T. spiralis larvae E/S Ags/Ca-BTC MOFs were used in prophylactic and therapeutic groups to either precede or follow the development of murine colitis. On the seventh day after colitis, mice were slaughtered. The effect of our target antigens on the progress of the colitis was evaluated using a variety of measures, including survival rate, disease activity index, colon weight/bodyweight, colon weight/length) ratios, and ratings for macroscopic and microscopic colon damage. The levels of inflammatory cytokines (interferon-γ and interleukin-4), oxidative stress marker malondialdehyde, and glutathione peroxidase in serum samples were evaluated. Foxp3 T-reg expression was carried out in colonic and splenic tissues. Results: T. spiralis larvae E/S Ags/Ca-BTC MOFs were the most effective in alleviating severe inflammation in murine colitis. The survival rate, disease activity index score, colon weight/length and colon weight/bodyweight ratios, and gross and microscopic colon damage scores have all considerably improved. A large decrease in proinflammatory cytokine (interferon-γ) and oxidative stress marker (malondialdehyde) expression and a significant increase in interleukin-4 and glutathione peroxidase expression were obtained. The expression of Foxp3+ Treg cells was elevated in colonic and splenic tissues. Conclusion: T. spiralis larvae E/S Ags/Ca-BTC MOFs had the highest anti-inflammatory, antioxidant, and cytoprotective capabilities against murine colitis and might be used to develop new preventative and treatment strategies.

Keywords

IBDT. spiralisExcretory-secretory antigensMOFsGSH-Px
Type
Research Paper
Information
Journal of Helminthology , Volume 98 , 2024 , e41
Copyright
© The Author(s), 2024. Published by Cambridge University Press

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