Hostname: page-component-76fb5796d-5g6vh Total loading time: 0 Render date: 2024-04-27T16:16:16.668Z Has data issue: false hasContentIssue false
  • English
  • Français

Studies on the infectivity of Diplostomum spathaceum in rainbow trout (Oncorhynchus mykiss)

Published online by Cambridge University Press:  05 June 2009

S. K. Whyte ,
C. J. Secombes  and
L. H. Chappell
S. K. Whyte
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB9 2TN, UK
C. J. Secombes
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB9 2TN, UK
L. H. Chappell*
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB9 2TN, UK
*
*Author for correspondence.
Get access Rights & Permissions [Opens in a new window]

Abstract

The infectivity of Diplostomum spathaceum (Digenea: Trematoda) cercariae to rainbow trout and the efficacy of the diplostomule migration to the lens following different routes of administration was examined. The optimum age of infectivity for cercariae was between 0–5 h after liberation from the snail and for intraperitoneally injected diplostomules, 5 h post-transformation in vitro through fish skin. After exposure of the entire fish body or head to cercariae, metacercariae first appeared in the lens at 5 h and their numbers gradually increased until 22 h. Following exposure of the tail region of rainbow trout to cercariae, metacercariae first appeared in the lens at 14 h. Significantly more metacercariae established in the lens of fish following exposure of the fish head compared with the tail region; 40% of penetrating cercariae reached the lens of fish following exposure of the head or entire body, 20% of cercariae or diplostomules injected either intraperitoneally, intramuscularly or intracardially reached the lens while only 5% of cercariae established as metacercariae following exposure of the tail region.

Keywords

Diplostomum spathaceumcercariaediplostomulesmigrationinfectivityTrematoda
Type
Research Papers
Information
Journal of Helminthology , Volume 65 , Issue 3 , September 1991 , pp. 169 - 178
Copyright
Copyright © Cambridge University Press 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Betterton, C. (1974) Studies on the host-specificity of the eyefluke, Diplostomum spathaceum in brown and rainbow trout. Parasitology, 69, 11–29.CrossRefGoogle Scholar
Chappell, L. H. (1967) Ecological and experimental studies on the parasites of freshwater fishes in Northern England. Ph.D. Thesis, University of Leeds.Google Scholar
Christensen, N. Ø. (1978) A method for the in vivo labelling of Diplostomum spathaceum, Hypoderaeum conoideum, Plagiorchiidae sp. and Notocotylus attenuatus cercariae with radioselenium. Zeitschrift für Parasitenkunde, 57, 155–162.CrossRefGoogle Scholar
Clegg, J. A. & Smithers, S. R. (1972) The effects of the immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. International Journal of Parasitology, 2, 79–98.Google Scholar
Erasmus, D. A. (1959) The migration of Cercaria X Baylis (Strigeida) within the intermediate fish host. Parasitology, 49, 173–190.Google Scholar
Gaten, E. (1987) Aggregation of the eyefluke Diplostomum spathaceum (Digenea: Diplostomatidae) in the lenses of various species of fish. Journal of Fish Diseases, 10, 69–74.CrossRefGoogle Scholar
Hoglund, J. (1990) Thermal effects on parasitic eyeflukes in fish—a case study. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science, 296.Google Scholar
Hughes, G. M. (1984) General anatomy of the gill. In: Fish Physiology, volume 10, Part A (editors Hoar, W. S. & Randall, D. J.) Published Academic Press, London pp. 1–72.Google Scholar
Johnson, K. A. (1971) The migration of Cotylurus erraticus cercariae (Trematoda: Strigeidae) in rainbow trout (Salmo gairdneri) and their effects on the host. Journal of Parasitology, 57, 244–251.CrossRefGoogle Scholar
McInnis, A. & Voge, M. (1970) Experiments and Techniques in Parasitology. W. H. Freeman & Co., San Fransisco, 232 pp.Google Scholar
van Oordt, B. (1987) The energy metabolism of the blood fluke Schistosoma mansoni. Ph.D. Thesis, University of Utrecht, The Netherlands.Google Scholar
Phillips, S. M., Reid, W. A. & Sadun, E. H. (1977) The cellular and humoral immune response to Schistosoma mansoni infections with inbred rats. II Mechanisms during re-exposure. Cellular Immunology, 28, 75–89.Google Scholar
Ratanarat-Brockelman, C. (1974) Migration of Diplostomum spathaceum (Trematoda) in the fish intermediate host. Zeitschrift für Parasitenkunde, 43, 123–134.Google Scholar
Reid, W. A., Phillips, S. M. & Roscinski, R. J. (1977) Schistosoma mansoni: Radioisotope uptake and retention by cercariae and developing schistosomules. Experimental Parasitology, 42, 331–342.Google Scholar
Shariff, M., Richards, R. H. & Sommerville, C. (1980) The histopathology of acute and chronic infections of rainbow trout, Salmo gairdneri (Richardson) with eyefluke, Diplostomum spp. Journal of Fish Diseases, 3, 455–465.Google Scholar
Stables, J. N. (1984) Studies on the speciation, epidemiology and immunology of Diplostomum spathaceum in fish. Ph.D. Thesis, University of Aberdeen.Google Scholar
Stables, J. N. & Chappell, L. H. (1986) Diplostomum spathaceum (Rud. 1819): effects of physical factors on the infection of rainbow trout, Salmo gairdneri, to Diplostomum spathaceum infections. Parasitology, 93, 71–79.Google Scholar
Whyte, S. K. (1989) Diplostomum spathaceum (Digenea) in rainbow trout; experimental and immunological studies, Ph.D. Thesis. University of Aberdeen.Google Scholar
Whyte, S. K., Chappell, L. H. & Secombes, C. J. (1988) In vitro transformation of Diplostomum spathaceum (Digenea) and short-term maintenance of post-penetration larvae in vitro. Journal of Helminthology, 62, 293–302.Google Scholar
Whyte, S. K., Chappell, L. H. & Secombes, C. J. (1990) Protection of rainbow trout (Salmo gairdneri) against Diplostomum spathaceum (Digenea): The role of specific antibody and activated macrophages. Journal of Fish Diseases, 13, 281–291.CrossRefGoogle Scholar