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Expression of substance P, vasoactive intestinal peptide and heat shock protein 70 in nasal mucosal smears of patients with allergic rhinitis: investigation using a liquid-based method

Published online by Cambridge University Press:  19 February 2008

T Chalastras ,
P Nicolopoulou-Stamati ,
E Patsouris ,
A Eleftheriadou ,
D Kandiloros ,
I Yiotakis ,
M Gonidi  and
P Athanassiadou
T Chalastras*
Affiliation:
Department of Pathology Medical School, University of Athens, Athens, Greece
P Nicolopoulou-Stamati
Affiliation:
Department of Pathology Medical School, University of Athens, Athens, Greece
E Patsouris
Affiliation:
Department of Pathology Medical School, University of Athens, Athens, Greece
A Eleftheriadou
Affiliation:
Department of Otolaryngology, Medical School, University of Athens, Hippokration Hospital, Athens, Greece
D Kandiloros
Affiliation:
Department of Otolaryngology, Medical School, University of Athens, Hippokration Hospital, Athens, Greece
I Yiotakis
Affiliation:
Department of Otolaryngology, Medical School, University of Athens, Hippokration Hospital, Athens, Greece
M Gonidi
Affiliation:
Department of Pathology Medical School, University of Athens, Athens, Greece
P Athanassiadou
Affiliation:
Department of Pathology Medical School, University of Athens, Athens, Greece
*
Address for correspondence: Dr Thomas Chalastras, Tenarou 35, Glyfada-Athens, GreecePC 16562. Fax: +302107223033 E-mail: chalastras@hotmail.com
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Abstract

Objective:

The aim of this study was to investigate expression of the neuropeptides substance P, vasoactive intestinal peptide and heat shock protein 70 in the nasal mucosa cells of patients with seasonal allergic rhinitis, in order to obtain more information on the pathophysiological and immunological role of these markers in allergic rhinitis.

Material and methods:

Nasal epithelium specimens obtained from 42 patients with allergic rhinitis were studied, using Shandon's Papspin liquid-based cytology method. Smears were immunostained with antibodies against substance P, vasoactive intestinal peptide and heat shock protein 70, and the results were correlated with the clinical features of seasonal allergic rhinitis.

Results:

A positive reaction for substance P, vasoactive intestinal peptide and heat shock protein 70 was observed in 73.8, 66.7 and 69.0 per cent of the allergic rhinitis mucosal smears, respectively. The Pearson chi-square test showed that 40.5 per cent of the immunostained smears had a positive reaction for one or two of the markers studied (i.e. substance P, vasoactive intestinal peptide or heat shock protein 70), and that 47.6 per cent of the smears had a positive reaction for all the markers (p < 0.0001).

Conclusions:

We found a high level of expression of substance P and vasoactive intestinal peptide in the nasal mucosa smears of patients suffering from allergic rhinitis. This indicates a role for these neuropeptides in the neuroregulation of immunity and hypersensivity in this disease. Furthermore, expression of heat shock protein 70 may contribute to the development of allergic rhinitis.

Keywords

Seasonal Allergic RhinitisNeuropeptidesVIP ReceptorsSubstance P
Type
Main Article
Information
The Journal of Laryngology & Otology , Volume 122 , Issue 7 , July 2008 , pp. 700 - 706
Copyright
Copyright © JLO (1984) Limited 2008

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