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Effects of Clitoria ternatea Leaf Extract on Growth and Morphogenesis of Aspergillus niger

Published online by Cambridge University Press:  03 July 2009

L. Kamilla
Centre for Drug Research, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
S.M. Mansor
Centre for Drug Research, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
S. Ramanathan
Centre for Drug Research, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
S. Sasidharan*
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
Corresponding author. E-mail:
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Clitoria ternatea is known for its antimicrobial activity but the antifungal effects of leaf extract on growth and morphogenesis of Aspergillus niger have not been observed. The extract showed a favorable antifungal activity against A. niger with a minimum inhibition concentration 0.8 mg/mL and minimum fungicidal concentration 1.6 mg/mL, respectively. The leaf extract exhibited considerable antifungal activity against filamentous fungi in a dose-dependent manner with 0.4 mg/mL IC50 value on hyphal growth of A. niger. The main changes observed under scanning electron microscopy after C. ternatea extract treatment were loss of cytoplasm in fungal hyphae and the hyphal wall and its diameter became markedly thinner, distorted, and resulted in cell wall disruption. In addition, conidiophore alterations were also observed when A. niger was treated with C. ternatea leaf extract.

Biological Applications
Copyright © Microscopy Society of America 2009

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