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Effects of Tributyltin Chloride on Developing Mouse Oocytes and Preimplantation Embryos

Published online by Cambridge University Press:  22 April 2015

Xian-Ju Huang ,
Ming Shen ,
Lizhong Wang ,
Fengxiang Yu ,
Wangjun Wu  and
Hong-lin Liu
Xian-Ju Huang
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Weigang No. 1, Nanjing 210095, China
Ming Shen
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Weigang No. 1, Nanjing 210095, China
Lizhong Wang
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Weigang No. 1, Nanjing 210095, China
Fengxiang Yu
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Weigang No. 1, Nanjing 210095, China
Wangjun Wu
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Weigang No. 1, Nanjing 210095, China
Hong-lin Liu*
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Weigang No. 1, Nanjing 210095, China
*
*Corresponding author. liuhonglin@njau.edu.cn
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Abstract

Tributyltin, an organotin, is ubiquitous in estuaries and freshwater systems. Previous reports suggest that tributyltin is an endocrine disruptor in many wildlife species and it inhibits aromatase in mammalian placental and granulosa-like tumor cell lines. However, no evidence showing the effects of tributyltin on oocytes or preimplantation embryonic developmental competence exists. Therefore, we investigated the role of tributyltin chloride (TBTCl) in the development of female oocytes and preimplantation embryos. Briefly, female ICR mice were gavaged with 0 (vehicle), 4, and 8 mg/kg of TBTCl each day for 18 days. The fluorescence intensity analysis showed that the 5-methylcytosine level decreased after TBTCl treatment, indicating that the general DNA methylation level decreased in the treated oocytes. Our results demonstrate that TBTCl treatment results in decreased mRNA levels of imprinted genes H19, Igf2r, and Peg3 during oocyte growth. The TBTCl-treated oocytes showed a significant increase in reactive oxygen species levels in germinal vesicle oocytes. In TBTCl-treated oocytes, there was no difference in GPx and Sod1 expression, but a decreased mRNA level of Cat occurred when compared with control. Moreover, the blastocysts with TBTCl exposure displayed higher apoptotic signals. These results suggest that TBTCl induces developmental defects in oocytes and preimplantation embryos.

Keywords

tributyltin chlorideDNA methylationROSdefects
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 2 , April 2015 , pp. 358 - 367
Copyright
© Microscopy Society of America 2015 

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