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Microanalysis Characterization of Bioactive Protein-Bound Polysaccharides Produced by Amanita Ponderosa Cultures

Published online by Cambridge University Press:  25 September 2014

Cátia Salvador ,
M. Rosário Martins  and
A. Teresa Caldeira
Cátia Salvador
Affiliation:
Chemistry Department, University of Évora, 7000-671 Évora, Portugal Chemistry Centre of Évora, University of Évora, 7000-671 Évora, Portugal Hercules Laboratory, University of Évora, 7000-809 Évora, Portugal
M. Rosário Martins
Affiliation:
Chemistry Department, University of Évora, 7000-671 Évora, Portugal Institute of Mediterranean Agricultural and Environmental Sciences, University of Évora, 7002-554 Évora, Portugal Hercules Laboratory, University of Évora, 7000-809 Évora, Portugal
A. Teresa Caldeira*
Affiliation:
Chemistry Department, University of Évora, 7000-671 Évora, Portugal Chemistry Centre of Évora, University of Évora, 7000-671 Évora, Portugal Hercules Laboratory, University of Évora, 7000-809 Évora, Portugal
*
*Corresponding author.atc@uevora.pt
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Abstract

Different compounds of edible mushrooms are responsible for their bioactivity. The ability to synthesize polysaccharides, namely protein–polysaccharide (PPS) complexes, is related to the antioxidant capacity of these compounds and present great interest in preventing a number of diseases, including cancer, cardiovascular and auto-immune diseases, and accelerated aging. Amanita ponderosa are wild edible mushrooms that grow in Mediterranean “montado” areas [Portuguese name given to cork oak (Quercus suber) and holm oak (Quercus ilex) forests]. The aim of this study was to evaluate the production of PPS complexes obtained from A. ponderosa cultures using a new microanalytical approach to quickly and easily monitor the production process. Microanalysis using Fourier-transform infrared using attenuated total reflection and Raman spectroscopy of PPS samples showed spectra compatible with identification of this type of compound in culture extracts. PPS separated by size-exclusion chromatography showed seven main complexes. Molecular weights of the main PPS complexes isolated from cultures ranged between 1.5 and 20 kDa and did not present toxicity against Artemia salina, demonstrating the potential of A. ponderosa as a source of biologically active compounds with nutraceutical value. Application of this microanalytical approach to monitoring the production of PPS compounds can be successfully applied in biotechnological processes.

Keywords

Amanita ponderosaedible mushroomsprotein-bound polysaccharidesFTIR-ATRRamansize-exclusion chromatography
Type
SPMicros Special Section
Information
Microscopy and Microanalysis , Volume 21 , Issue 1 , February 2015 , pp. 84 - 90
Copyright
© Microscopy Society of America 2014 

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