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Biodegradation Process of α-Tricalcium Phosphate and α-Tricalcium Phosphate Solid Solution Bioceramics In Vivo: A Comparative Study

Published online by Cambridge University Press:  03 July 2013

Piedad N. de Aza ,
Zofia B. Luklinska ,
Jose E. Mate-Sanchez de Val  and
Jose L. Calvo-Guirado
Piedad N. de Aza*
Affiliation:
Instituto de Bioingeniería, Universidad Miguel Hernández, Avda. Universidad s/n, 03202 Elche (Alicante), Spain
Zofia B. Luklinska
Affiliation:
Materials Science Department, SEMS, Queen Mary University of London, Mile End Road London E1 4NS, UK
Jose E. Mate-Sanchez de Val
Affiliation:
Department of Restorative Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Ava. Marques de los Velez s/n, 30008 Murcia, Spain
Jose L. Calvo-Guirado
Affiliation:
Department of Implant Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Ava. Marques de los Velez s/n, 30008 MurciaSpain
*
*Corresponding author. E-mail: piedad@umh.es
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Abstract

This article reports the structure and morphology of the in vivo interface between implants composed of either α tricalcium phosphate (αTCP) or αTCP doped with 3.0 wt% dicalcium silicate (αTCPss) ceramic, and natural bone of rabbit tibias. Both interfaces developed a new bone layer in direct contact with the implants after 4 and 8 weeks of implantation. The specimens were examined using analytical scanning and transmission electron microscopy, up to the lattice plane resolution level. Degradation processes of the implants developed at the interfaces encouraged osseous tissue ingrowth into the periphery of the material, changing the microstructure of the implants. The ionic exchange initiated at the implant interface with the environment was essential in the integration process of the implant, through a dissolution–precipitation–transformation mechanism. The interfaces developed normal biological and chemical activities and remained reactive over the 8-week period. Organized collagen fibrils were found at the αTCPss/bone interface after 4 weeks, whereas a collagen-free layer was present around the Si-free αTCP implants. These findings suggest that the incorporation of silicate ions into αTCP ceramic promotes processes of the bone remodeling at the bone/αTCPss interface, hence the solubility rate of the αTCPss material decreased.

Keywords

biocompatibilitybiodegradationin vivoelectron microscopysilicatecalcium phosphate
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 5 , October 2013 , pp. 1350 - 1357
Copyright
Copyright © Microscopy Society of America 2013 

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