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Combined Anastrozole and Antiplatelet Therapy Treatment Differentially Promotes Breast Cancer Cell Survival

Published online by Cambridge University Press:  03 April 2020

Kutlwano Xulu Open the ORCID record for Kutlwano Xulu [Opens in a new window] ,
Raquel Duarte  and
Tanya Augustine
Kutlwano Xulu*
Affiliation:
School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, 2193Johannesburg, South Africa
Raquel Duarte
Affiliation:
Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, 2193Johannesburg, South Africa
Tanya Augustine*
Affiliation:
School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, 2193Johannesburg, South Africa
*
*Authors for correspondence: Tanya Augustine, E-mail: Tanya.Augustine@wits.ac.za; Kutlwano Xulu, E-mail: kutlwano.xulu@wits.ac.za
*Authors for correspondence: Tanya Augustine, E-mail: Tanya.Augustine@wits.ac.za; Kutlwano Xulu, E-mail: kutlwano.xulu@wits.ac.za
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Abstract

Thromboembolic disorders are the second leading cause of death in breast cancer. Antiplatelet therapy combined with cancer therapy is a potential treatment strategy against cancer-associated thromboembolic disorders; however, the efficacy of such dual treatment has not been established. This study reports novel findings on the response of hormone-dependent breast cancer cell lines (MCF7/T47D) following 24 h treatment with Anastrozole, combined with Aspirin and Clopidogrel cocktail; and Atopaxar. Neutral red and lactate dehydrogenase assays were conducted to assess viability and cytotoxicity respectively. Flow cytometric Annexin-V/PI assay was used to assess the mode of cell death. Morphological alterations were studied using scanning electron microscopy. Statistical analysis was conducted using Statistica V13. Definitive outcomes were established with flow cytometric detection of phosphatidylserine exposure and propidium iodide staining, complemented with ultrastructural analysis. Results showed that a few cells were undergoing death mainly through secondary necrosis. Morphological features suggesting induced cell motility (pseudopodia/ruffled membranes) were observed in both cell lines; notably, T47D cells presented pronounced features than MCF7 cells. Overall, these findings suggest that such combined treatment may differentially promote cell survival, inducing a more aggressive breast cancer phenotype.

Keywords

anastrozoleantiplatelet therapybreast cancercell survival
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 26 , Issue 3 , June 2020 , pp. 497 - 508
Copyright
Copyright © Microscopy Society of America 2020

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Footnotes

*The author names and corresponding author information have been corrected since publication. A corrigendum notice detailing the changes has also been published: https://doi.org/10.1017/S1431927620001622.

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