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Development of Rapid Tilt-Series Collection for Electron Cryo-Tomography

Published online by Cambridge University Press:  05 August 2019

Georges Chreifi ,
Songye Chen ,
Lauren Ann Metskas ,
Mohammed Kaplan ,
Catherine M. Oikonomou  and
Grant J. Jensen
Georges Chreifi
Affiliation:
Div. of Biology and Biological Engineering, California Inst. of Tech., Pasadena, CA 91125, USA.
Songye Chen
Affiliation:
Div. of Biology and Biological Engineering, California Inst. of Tech., Pasadena, CA 91125, USA.
Lauren Ann Metskas
Affiliation:
Div. of Biology and Biological Engineering, California Inst. of Tech., Pasadena, CA 91125, USA. Howard Hughes Medical Institute, Pasadena, CA 91125, USA.
Mohammed Kaplan
Affiliation:
Div. of Biology and Biological Engineering, California Inst. of Tech., Pasadena, CA 91125, USA.
Catherine M. Oikonomou
Affiliation:
Div. of Biology and Biological Engineering, California Inst. of Tech., Pasadena, CA 91125, USA.
Grant J. Jensen*
Affiliation:
Div. of Biology and Biological Engineering, California Inst. of Tech., Pasadena, CA 91125, USA. Howard Hughes Medical Institute, Pasadena, CA 91125, USA.
*
*Presenting and corresponding author: jensen@caltech.edu

Abstract

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Type
Advances in Cryo-EM Technology
Information
Microscopy and Microanalysis , Volume 25 , Supplement S2 , August 2019 , pp. 988 - 989
Copyright
Copyright © Microscopy Society of America 2019 

References

[1]Chreifi, G. et al. , Journal of Structural Biology 205 (2019), pp. 163-169.Google Scholar
[2]Schur, F. K. et al. , Science 353 (2016), pp. 506-8.Google Scholar
[3]Himes, B. A. and Zhang, P., Nature Methods 15 (2018), pp. 955-961.Google Scholar
[4]Bharat, T. A. M. et al. , Nature Microbiology 2 (2017), 17059.Google Scholar
[5]Hegerl, R. and Hoppe, W., Zeitschrift fur Naturforschung A 31 (1976), pp. 1717-1721.Google Scholar
[6]Song, K. et al. , bioRxiv, 363317 (2018), https://doi.org/10.1101/363317 (accessed 2019 Jan 12]Google Scholar
[7]The authors thank David N. Mastronarde for helpful discussion. This work was supported by the NIH (grant GM122588 to G. J. J.) and de Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO postdoctoral Rubicon fellowship to M. K.). Electron cryomicroscopy was done in the Beckman Institute Resource Center for Transmission Electron Microscopy at Caltech.Google Scholar