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Epithelial Distribution of E-Cadherin, p63, and Mitotic Figures in ApoTome Images to Determine the Oncogenic Potentiality of Oral Submucous Fibrosis

Published online by Cambridge University Press:  14 October 2020

Reetoja Nag ,
Ranjan Rashmi Paul ,
Mousumi Pal ,
Jyotirmoy Chatterjee  and
Raunak Kumar Das
Reetoja Nag*
Affiliation:
Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology, Room No. 402, ALM Building, Vellore 632014, Tamil Nadu, India
Ranjan Rashmi Paul
Affiliation:
Gurunanak Institute of Dental Sciences and Research, Panihati 700114, West Bengal, India
Mousumi Pal
Affiliation:
Gurunanak Institute of Dental Sciences and Research, Panihati 700114, West Bengal, India
Jyotirmoy Chatterjee
Affiliation:
School of Medical Science and Technology, Indian Institute of Technology, Kharagpur 721302, West Bengal, India
Raunak Kumar Das*
Affiliation:
Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology, Room No. 402, ALM Building, Vellore 632014, Tamil Nadu, India
*
*Author for correspondence: Reetoja Nag, E-mail: nagreetoja@gmail.com; and Raunak Kumar Das E-mail: raunakkumardas@vit.ac.in
*Author for correspondence: Reetoja Nag, E-mail: nagreetoja@gmail.com; and Raunak Kumar Das E-mail: raunakkumardas@vit.ac.in
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Abstract

The exact process of the malignant conversion of oral submucous fibrosis (OSF) to oral cancer is not fully understood. This study aimed to detect and analyze E-cadherin expression, p63 expression, and number of mitotic figures, all correlated to cancer development, in ApoTome images of oral tissues to determine the oncogenic potentiality of OSF. ApoTome images of the study groups (6 normal, 16 OSF with dysplasia, and 10 OSF without dysplasia) were recorded. Cytoplasmic and membranous E-cadherin expression, breakages of the cell membrane, and p63 expression were detected in MATLAB 2016b. The number of mitotic figures detected by MATLAB was correlated with the number of chromosomes detected by ImageJ. A Mann–Whitney U test was done to determine a significant difference between the study groups for cytoplasmic and membranous E-cadherin distribution points. Statistical significant differences were found for cytoplasmic E-cadherin distribution between normal and OSF (with dysplasia) (p = 0.0278). There was an increase in mitotic figures, p63 expression, and cytoplasmic E-cadherin expression and a decrease in membranous E-cadherin expression from normal to diseased condition. Hence, automated detection and quantification of E-cadherin, p63, and mitotic figures in ApoTome images of oral biopsies can help in determining the oncogenic potentiality of OSF.

Keywords

ApoTomeE-cadherinmitotic figuresoral submucous fibrosisp63
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 26 , Issue 6 , December 2020 , pp. 1198 - 1210
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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