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Fibrin and Transforming Growth Factor Alpha Affect Prostatic Smooth Muscle Cell's Phenotype and Motility

Published online by Cambridge University Press:  11 March 2021

Daniel A. Osório ,
Silvio R. Consonni ,
Aline M. dos Santos  and
Hernandes F. Carvalho
Daniel A. Osório
Affiliation:
Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil
Silvio R. Consonni
Affiliation:
Department of Biochemistry and Tissue Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil
Aline M. dos Santos
Affiliation:
Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil National Institute of Photonics Applied to Cell Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil
Hernandes F. Carvalho*
Affiliation:
Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil National Institute of Photonics Applied to Cell Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil
*
*Author for correspondence: Hernandes F. Carvalho, E-mail: hern@unicamp.br
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Abstract

Smooth muscle cells (SMCs) are dynamic and transition from a contractile to a synthetic phenotype under different circumstances. Plasma factors (fibrin and transforming growth factors, TGFs) are possible components affecting SMCs differentiation and behavior. Thus, the objective of this work was to investigate how the fibrin matrix and TGFs affect SMCs differentiation and motility behavior. SMCs invaded the fibrin gel and adopted a stellate phenotype while reducing the expression of differentiation markers (Acta2, Myh11, and Smtn). At the ultrastructural level, SMCs did not assemble a basal lamina and showed numerous blebs along the entire cell surface. This transition was not associated with changes in focal adhesion kinase (FAK) content and phosphorylation status but reflected a marked change in FAK distribution in the cytoplasm. After 48 h in culture, SMCs caused an active degradation of the fibrin gel. Additionally, we tested the SMCs response to TGFs in a cell layer wound repair assay. TGFα, but not TGFβ1 or TGFβ3, had significantly increased motility. In conclusion, prostatic SMCs present a phenotypical transition when cultured on fibrin, adopting a micro-blebbing based motility behavior and increasing migration in response to TGFα.

Keywords

α-smooth muscle actinFAKfibrinprostatesmooth muscle cellsTGFα
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 27 , Issue 3 , June 2021 , pp. 579 - 586
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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