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Filamentous versus Spherical Morphology: A Case Study of the Recombinant A/WSN/33 (H1N1) Virus

Published online by Cambridge University Press:  10 February 2020

Larisa V. Kordyukova Open the ORCID record for Larisa V. Kordyukova [Opens in a new window] ,
Ramil R. Mintaev ,
Artyom A. Rtishchev ,
Marina S. Kunda ,
Natalia N. Ryzhova ,
Sergei S. Abramchuk ,
Marina V. Serebryakova ,
Vladislav V. Khrustalev ,
Tatyana A. Khrustaleva  and
Victor V. Poboinev
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Larisa V. Kordyukova*
Affiliation:
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991Moscow, Russia
Ramil R. Mintaev
Affiliation:
Mechnikov Research Institute of Vaccine and Sera, 105064Moscow, Russia Federal State Budgetary Institution «Center for Strategic Planning and Management for Medical and Biological Health Risks», Ministry of Health, 119121Moscow, Russia
Artyom A. Rtishchev
Affiliation:
Mechnikov Research Institute of Vaccine and Sera, 105064Moscow, Russia
Marina S. Kunda
Affiliation:
N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health, 123098Moscow, Russia
Natalia N. Ryzhova
Affiliation:
N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health, 123098Moscow, Russia
Sergei S. Abramchuk
Affiliation:
Department of Chemistry, Lomonosov Moscow State University, 119234Moscow, Russia A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, 119991Moscow, Russia
Marina V. Serebryakova
Affiliation:
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991Moscow, Russia
Vladislav V. Khrustalev
Affiliation:
Department of General Chemistry, Belarusian State Medical University, 220116Minsk, Belarus
Tatyana A. Khrustaleva
Affiliation:
Biochemical Group of the Multidisciplinary Diagnostic Laboratory, Institute of Physiology of the National Academy of Sciences of Belarus, 220072Minsk, Belarus
Victor V. Poboinev
Affiliation:
Department of General Chemistry, Belarusian State Medical University, 220116Minsk, Belarus
Stanislav G. Markushin
Affiliation:
Mechnikov Research Institute of Vaccine and Sera, 105064Moscow, Russia
Olga L. Voronina
Affiliation:
N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health, 123098Moscow, Russia
*
*Author for correspondence: Larisa V. Kordyukova, E-mail: kord@belozersky.msu.ru
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Abstract

Influenza A virus is a serious human pathogen that assembles enveloped virions on the plasma membrane of the host cell. The pleiomorphic morphology of influenza A virus, represented by spherical, elongated, or filamentous particles, is important for the spread of the virus in nature. Using fixative protocols for sample preparation and negative staining electron microscopy, we found that the recombinant A/WSN/33 (H1N1) (rWSN) virus, a strain considered to be strictly spherical, may produce filamentous particles when amplified in the allantoic cavity of chicken embryos. In contrast, the laboratory WSN strain and the rWSN virus amplified in Madin–Darby canine kidney cells exhibited a spherical morphology. Next-generation sequencing (NGS) suggested a rare Ser126Cys substitution in the M1 protein of rWSN, which was confirmed by the mass spectrometric analysis. No structurally relevant substitutions were found by NGS in other proteins of rWSN. Bioinformatics algorithms predicted a neutral structural effect of the Ser126Cys mutation. The mrWSN_M1_126S virus generated after the introduction of the reverse Cys126Ser substitution exhibited a similar host-dependent partially filamentous phenotype. We hypothesize that a shortage of some as-yet-undefined cellular components involved in virion budding and membrane scission may result in the appearance of filamentous particles in the case of usually “nonfilamentous” virus strains.

Keywords

electron microscopy protocolsfilamentous virus particlesinfluenza A virusM1 matrix proteinnext-generation sequencing
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 26 , Issue 2 , April 2020 , pp. 297 - 309
Copyright
Copyright © Microscopy Society of America 2020

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