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Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats

Published online by Cambridge University Press:  10 June 2020

Jasmina Sretenovic ,
Vladimir Zivkovic ,
Ivan Srejovic ,
Vladimir Ajdzanovic ,
Natasa Ristic ,
Miroljub Trifunovic ,
Suzana Pantovic ,
Slavoljub Jovic ,
Vladimir Jakovljevic  and
Sergey Bolevich
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Jasmina Sretenovic
Affiliation:
Faculty of Medical Sciences, Department of Physiology, University of Kragujevac, Svetozara Markovica 69, 34000Kragujevac, Serbia
Vladimir Zivkovic
Affiliation:
Faculty of Medical Sciences, Department of Physiology, University of Kragujevac, Svetozara Markovica 69, 34000Kragujevac, Serbia
Ivan Srejovic*
Affiliation:
Faculty of Medical Sciences, Department of Physiology, University of Kragujevac, Svetozara Markovica 69, 34000Kragujevac, Serbia
Vladimir Ajdzanovic
Affiliation:
Department of Cytology, Institute for Biological Research “Sinisa Stankovic” – National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000Belgrade, Serbia
Natasa Ristic
Affiliation:
Department of Cytology, Institute for Biological Research “Sinisa Stankovic” – National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000Belgrade, Serbia
Miroljub Trifunovic
Affiliation:
Department of Otorhinolaryngology and Maxillofacial Surgery, General Hospital Studenica, Jug Bogdanova 110, 36000Kraljevo, Serbia
Suzana Pantovic
Affiliation:
Faculty of Medical Sciences, Department of Physiology, University of Kragujevac, Svetozara Markovica 69, 34000Kragujevac, Serbia
Slavoljub Jovic
Affiliation:
Faculty of Veterinary Medicine, Department of Physiology and Biochemistry, University of Belgrade, Bulevar oslobodjenja 18, Belgrade11000, Serbia
Vladimir Jakovljevic
Affiliation:
Faculty of Medical Sciences, Department of Physiology, University of Kragujevac, Svetozara Markovica 69, 34000Kragujevac, Serbia Department of Human Pathology, I.M. Sechenov First Moscow State Medical University, Moscow119992, Russian Federation
Sergey Bolevich
Affiliation:
Department of Human Pathology, I.M. Sechenov First Moscow State Medical University, Moscow119992, Russian Federation
Zoran Milosavljevic
Affiliation:
Faculty of Medical Science, Department of Histology and Embryology, University of Kragujevac, Svetozara Markovica 69, 34 000Kragujevac, Serbia
Verica Milosevic
Affiliation:
Department of Cytology, Institute for Biological Research “Sinisa Stankovic” – National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000Belgrade, Serbia
*
*Author for correspondence: Ivan Srejovic, E-mail: ivan_srejovic@hotmail.com
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Abstract

The aim of the study was to investigate the effects of chronic nandrolone decanoate treatment and/or swimming training on immunohistomorphometric parameters on rat pituitary gonadotropic cells. Male Wistar albino rats, 10 weeks old, were classified into four groups: control (T−N−), nandrolone (T−N+), swimming training (T+N−), and swimming training with nandrolone (T+N+). The T+ groups swam for 4 weeks, 1 h/day, 5 days/week. The N+ groups received nandrolone decanoate (20 mg/kg) once per week for 4 weeks. Pituitary tissue sections were processed and stained for immunohistochemical analysis and immunofluorescence. The volume density of luteinizing hormone (LH) cells was decreased by 48% in T−N+ and for 35% in the T+N+ group. The volume density of follicle-stimulating hormone (FSH) cells was decreased by 39% in T−N+ and for 30% in T+N+ compared to the control. Nandrolone alone, or combined with swimming training, decreased the number of LH/FSH cells compared to the control. The levels of the immunofluorescent signal of LH/FSH cells were increased in all experimental groups. Nandrolone alone decreased the serum level of LH by 17%, whereas swimming training alone increased FSH levels by 11% compared to the control. Serum levels of testosterone were increased in all experimental groups. Nandrolone alone, or combined with swimming training, decreased immunohistomorphometric parameters of gonadotropic cells, whereas the levels of immunofluorescent signal were increased.

Keywords

FSHLHnandrolonepituitaryswimmingtestosterone
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 26 , Issue 4 , August 2020 , pp. 699 - 707
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Copyright
Copyright © Microscopy Society of America 2020

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