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Impact of Glyphosate-Roundup® in the Ileal Structure of Male and Female Rats: A Morphological and Immunohistochemical Study

Part of: Micrographia Collection

Published online by Cambridge University Press:  22 September 2021

Shaimaa M.M. Saleh Open the ORCID record for Shaimaa M.M. Saleh [Opens in a new window] ,
Tasneem A. Elghareeb ,
Mona M. Atia  and
Mohamed Ahmed Ibrahim Ahmed
Shaimaa M.M. Saleh*
Affiliation:
Department of Zoology and Entomology, Faculty of Science, Assiut University, Assiut71516, Egypt
Tasneem A. Elghareeb
Affiliation:
Plant Protection Department, Faculty of Agriculture, Assiut University, Assiut71526, Egypt
Mona M. Atia
Affiliation:
Department of Zoology and Entomology, Faculty of Science, Assiut University, Assiut71516, Egypt
Mohamed Ahmed Ibrahim Ahmed
Affiliation:
Plant Protection Department, Faculty of Agriculture, Assiut University, Assiut71526, Egypt
*
*Corresponding author: Shaimaa M.M. Saleh, E-mail: shaimaasaleh@aun.edu.eg
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Abstract

The current study was aimed to evaluate the effects of variable doses of the weedicide glyphosate on the ileal (the final section of the small intestine) structure of rats of both sexes, using histological, histochemical, and ultrastructural methods. Forty animals were classified into four groups of 10 animals per group (five males and five females). The first group acted as a control, and the remaining groups were treated with glyphosate-Roundup® 25, 50, and 100 mg/kg body weight daily for 15 days. The results indicated extinct histopathological changes manifested in the deformation of villi, foci of leukocytic infiltration in the core of villi, and hyperplasia of goblet cells. Histochemical examination (Alcian blue and Periodic acid–Schiff stain) revealed a strong positive reaction of goblet cells and an increase in their number in all treated groups. In addition, the immunohistochemical investigation revealed the immunoreactivity of matrix metalloproteinase-9 expression. Furthermore, electron microscopic alternations were represented by the deformation of nuclei, destruction of microvilli, and deposition of lipid droplets. Collectively, the present findings indicate that treatment with glyphosate results in extensive morphological alternations to the ileal structure of rats of both sexes and that female rats are more affected than male rats are.

Keywords

glyphosatehistopathologyileal structureimmunohistochemistrymorphological alternations
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 27 , Issue 6 , December 2021 , pp. 1547 - 1563
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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