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Induction of Apoptic Features in Human Colon Cancer Cells After Exposure to Specific Vitamin Combinations

Published online by Cambridge University Press:  02 July 2020

B. Brown ,
K. R. Garvin ,
B. G. Hughes ,
M. D. Standing ,
K. L. O'Neill  and
B. K. Murray
B. Brown
Affiliation:
Department of Microbiology, Brigham Young University, Provo, Utah, 84602
K. R. Garvin
Affiliation:
Department of Microbiology, Brigham Young University, Provo, Utah, 84602
B. G. Hughes
Affiliation:
Plant Bioactives Research Institute, Orem, Utah, 84058
M. D. Standing
Affiliation:
Microscopy Laboratory, Brigham Young University, Provo, Utah, 84602
K. L. O'Neill
Affiliation:
Department of Microbiology, Brigham Young University, Provo, Utah, 84602
B. K. Murray
Affiliation:
Department of Microbiology, Brigham Young University, Provo, Utah, 84602
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Extract

Proper nutrition and vitamin consumption have long been associated with lower risks for many human cancers. Data from recent studies have suggested that exposure to antioxidant vitamins and vitamin combinations can induce genetic programed cell death, apoptosis, in human cancer cells. ‘“3 In this study, we report on the induction of apoptosis in HT-29 human colon adenocarcinoma cells after exposure to low doses of 13- c/s-retinoic acid (RA) and vitamin E succinate (VES). Induction of apoptosis was seen only in cancerous colon cells and not in normal human colon cells when exposed to RA and VES in combination, but not when exposed to either vitamin alone.

Cultures of HT-29 cells were seeded in 6 well plates at 10,000 cells/well and grown at 37°C, 5% C02 in RPMI 1640 medium supplemented with 10% fetal bovine serum.

Type
Biological Structure (Cells, Tissues, Organ Systems)
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 892 - 893
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Guzey, M. et al., Combinatorial effects of vitamin D3, and retinoic acid (all trans and 9 cis) on proliferation, differentiation, and programmed cell death in two small cell lung carcinoma cell lines. Biochem. Biophys. Res. Commun. 249 (1998) 735.CrossRefGoogle Scholar
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