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Morphological and Histochemical Changes in Macrophage Activity After Novel Anti-Neoplastic Platinum Agents

Published online by Cambridge University Press:  02 July 2020

H. J. Muenchen ,
S.K. Aggarwal ,
H.K. Misra  and
P. J. Andrulis
H. J. Muenchen
Affiliation:
Department of Zoology, Michigan State University, East Lansing, MI, 48824-1115
S.K. Aggarwal
Affiliation:
Department of Zoology, Michigan State University, East Lansing, MI, 48824-1115
H.K. Misra
Affiliation:
Andrulis Pharmaceuticals Corporation, Beltsville, Maryland, 20705
P. J. Andrulis
Affiliation:
Andrulis Pharmaceuticals Corporation, Beltsville, Maryland, 20705
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Extract

Poly-[(trans-1,2-diaminocyclohexane) platinumj-carboxyamylose (“poly-plat”), 5-sulfosalicylato-trans -(1,2-diaminocyclohexane) platinum (SSP), and 4-hydroxy-∝-sulfonylphenylacetato (trans 1,2-diaminocyclohexane) platinum (II) (SAP) are second generation analogs of cisplatin (CDDP) with higher efficacy and potency than cisplatin. This is particularly true of “poly-plat” which contains 1/5 the platinum of CDDP. In order to understand the mechanism of action of these compounds, isolated murine peritoneal macrophages in culture medium were treated with “poly-plat”, SSP, or SAP (5 μg/ml) for 2 h. Drug containing medium was then replaced with fresh medium and the cells were allowed to incubate at 37° C (5% CO2) for 24 h. Supernatants were collected at 0.5, 1, 2, and 24 h post-treatment for immunocytochemical analysis. Confocal microscopy studies demonstrated an increase in the number of lysosomes in the treated macrophages, but only “poly-plat” and SSP treated macrophages were stimulated to form cytoplasmic extensions at 2 h and 24 h.

Type
Neoplasia: Abnormal Cell Growth or Death/Apoptosis? Insights from Microscopy
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 11 - 12
Copyright
Copyright © Microscopy Society of America 1997

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References

1. Fiebig, H.H.et al., GB-21, a novel platinum polymer complex with antitumor activity in human renal and mammary xenografts. Proc. Amer. Cancer Res. 37(1996)297.Google Scholar

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