Hostname: page-component-848d4c4894-xfwgj Total loading time: 0 Render date: 2024-06-16T01:23:35.018Z Has data issue: false hasContentIssue false

New hardware for a streamlined cryo focused ion beam milling workflow

Published online by Cambridge University Press:  30 July 2021

Sebastian Tacke
Affiliation:
Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Otto-Hahn-Str. 11, 44227Dortmund, Germany, United States
Zhexin Wang
Affiliation:
Max Planck Institute of Molecular Physiology, Dortmund, Germany
Michael Grange
Affiliation:
Max Planck Institute of Molecular Physiology, Dortmund, Germany
Stefan Raunser
Affiliation:
Max Planck Institute of Molecular Physiology, United States

Abstract

Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
Cryo-electron Tomography: Present Capabilities and Future Potential
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Grange, M, Vasishtan, D., & Grünewald, K. Cellular electron cryo tomography and in situ sub-volume averaging reveal the context of microtubule-based processes. J. Struct. Biol. 197, 181-190 (2017).CrossRefGoogle ScholarPubMed
Bartesaghi, A. et al. Secondary Structure Determination by Constrained Single-Particle Cryo-Electron Tomography. Structure 20, 2003-2013 (2012).CrossRefGoogle ScholarPubMed
Schur, F. K. M. et al. An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation. Science 353, 506-508 (2016).CrossRefGoogle ScholarPubMed
Vulovic, M. et al. Image formation modelling in cryo-electron microscopy. J. Struct. Biol. 183, 19-32 (2013).CrossRefGoogle Scholar
Al-Alamoudi, A., Norlen, L.P.O., & Dubochet, J. Cryo-electron microscopy of vitreous sections of native biological cells and tissues. J. Struct. Biol. 148, 131-135 (2004)CrossRefGoogle Scholar
Al-Alamoudi, A., Studer, D. & Dubochet, J. Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. J. Struct. Biol. 150, 109-121 (2005).CrossRefGoogle Scholar
Marko, M. et al. Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy. Nat. Methods 4, 215-217 (2007).Google ScholarPubMed
Rigort, A. et al. Micromachining tools and correlative approaches for cellular cryo-electron tomography. J. Struct. Biol. 172, 169-179 (2010).Google ScholarPubMed
Mahamid, J. et al. Visualizing the molecular sociology at the HeLa cell nuclear periphery. Science 351, 969972 (2016).CrossRefGoogle ScholarPubMed
Medeiros, J.M. et al. Robust workflow and instrumentation for cryo-focused ion beam milling of samples for electron cryotomography. Ultramicroscopy 190, 1-11 (2018).CrossRefGoogle ScholarPubMed