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New Insights Into the Three-Dimensional Structure of the Nucleus and Chromosomes

Published online by Cambridge University Press:  02 July 2020

J. Sedat ,
W. Marshall ,
A. Dernburg ,
J. Fung  and
D. Agard
J. Sedat
Affiliation:
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA94143-0554
W. Marshall
Affiliation:
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA94143-0554
A. Dernburg
Affiliation:
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA94143-0554
J. Fung
Affiliation:
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA94143-0554
D. Agard
Affiliation:
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA94143-0554
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Extract

Recent methodology, to be described, now makes possible specific localization and analysis of genetic loci within 3-Dimensional interphase nuclei in intact cells and tissues with minimal perturbation of the chromosome structure (Dernburg and Sedat, 1997). These techniques define genetic loci that specifically interact with the nuclear envelope and interior structures; we are able to map all loci to highly localized 3-dimensional positions within Drosophila embryonic nuclei (Marshall et al, 1996). S-Dimensions-as-a-function-of-time (4-D) studies of live nuclei, from Yeast and Drosophila, allow dynamic chromosome interactions to be probed and quantitated. Our results suggest a very dynamic but highly determined and organized nucleus. Using these approaches, we can now study specific mechanisms leading to homologue chromosome pairing and position-effect variegation (Dernburg et al., 1996).

Type
Innovative Approaches to 3-D Structure/Function Determination for Cells and Organelles
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 213 - 214
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Dernburg, A. F. et al., Methods in Cell Biology, (1997) in press.Google Scholar
2.Marshall, W. F. et al., Mol. Biol. of the Cell, (1996)825.10.1091/mbc.7.5.825CrossRefGoogle Scholar
3.Dernburg, A. F. et al., Cell, (1996) 745.10.1016/S0092-8674(00)81240-4CrossRefGoogle Scholar