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Novel Polarizing Method for Light Microscopy

Published online by Cambridge University Press:  19 September 2016

Irina G. Palchikova ,
Evgenii S. Smirnov  and
Natalia V. Kamanina
Irina G. Palchikova*
Affiliation:
Technological Design Institute of Scientific Instrument Engineering, Siberian Branch, Russian Academy of Sciences, ul. Russkaya, 41, Novosibirsk, 630058Russia Novosibirsk State University, Department of Physics, ul. Pirogova, 2, Novosibirsk, 630090Russia
Evgenii S. Smirnov
Affiliation:
Technological Design Institute of Scientific Instrument Engineering, Siberian Branch, Russian Academy of Sciences, ul. Russkaya, 41, Novosibirsk, 630058Russia
Natalia V. Kamanina
Affiliation:
Vavilov State Optical Institute, Lab for Photophysics of Media with Nanoobjects, Kadetskaya Liniya Vasilyevskogo Ostrova, 5, Building 2, St. Petersburg, 199053Russia
*
*Corresponding author.palchikova@gmail.com
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Abstract

A test of the qualities of polarizing filters was performed for a set of specimens including a bulk Nicol prism, standard polaroids, and special polyvinyl alcohol (PVA)-iodine thin-film filters coated on both sides by vertically oriented carbon nanotubes. The residual transmission of polarizing filters depending on the incidence angle of polarized light was examined in detail. The superior quality of polarizing film filters treated with carbon nanotubes was found. This fact allows us to propose a new application for polarizing films with carbon nanotubes for a polarizing cover glass. In such a way the cover glass may serve as an analyzer in a light polarizing microscope. Some features of optical scheme arrangement for the polarizing technique are discussed. The polarizing cover glass allows elimination of depolarization of light, which is inserted in a microscope objective. Test results of the proposed polarizing technique attest to the efficiency of using the polarizing cover glass. The new scheme for polaroid arrangement shows image-contrast enhancement by several percent in comparison with the standard layout.

Keywords

nanotubespolarizing filmlight microscopypolarization techniquepolarizing cover glass
Type
Instrumentation and Techniques Development
Information
Microscopy and Microanalysis , Volume 22 , Issue 5 , October 2016 , pp. 933 - 938
Copyright
© Microscopy Society of America 2016 

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References

Ayupov, B.M. & Prokhorova, S.A. (1998). Detection of optic anisotropy in metallophtalocyanine films by the method of null ellipsometry. Optoelectron Instrum Data Process 2, 52–61.Google Scholar
Born, M. & Wolf, E. (1999). Principles of Optics Electromagnetic Theory of Propagation, Interference and Diffraction of Light. Cambridge: Cambridge University Press.Google Scholar
Kamanina, N.V., Omelyanchuk, L.V., Palchikova, I.G., Smirnov, E.S. & Chugui, Yu.V. (2011 a). Microscope cover glass. Patent RU 2436137 G02B 21/34.Google Scholar
Kamanina, N.V., Vasilyev, P.Ya. & Studeonov, V.I. (2010 a). Thin-film polarizers for visible spectral range with nanostructured surface modified by carbon nanotubes. Tech Phys Lett 36(8), 727–728.Google Scholar
Kamanina, N.V., Vasilyev, P.Ya. & Studeonov, V.I. (2011 b). Polarizing films for visible spectrum range with nanostructured surface based on carbon nanotubes. Patent RU 2426157 С1.Google Scholar
Kamanina, N.V., Vasilyev, P.Ya., Studeonov, V.I., Pogareva, V.G., Pavlova, V.A., Tupikov, V.A., Palchikova, I.G. & Chugui, Yu.V. (2010 b). Nanostructured materials for lasers and display applications. Proceedings of the Sixth International Conference on Mathematical Modeling and Computer Simulation of Materials Technologies (MMT-2010), Ariel University Center of Samaria, Ariel, Israel, August 23–27, 2010, paper 2–50.Google Scholar
Lacey, A.J. (Ed.) (1999). Light Microscopy in Biology: A Practical Approach. Oxford: Oxford University Press.CrossRefGoogle Scholar
Palchikova, I.G., Omelyanchuk, L.V., Kamanina, N.V., Makarov, S.N. & Smirnov, E.S. (2013). Finding fluorescence polarization anisotropy from digital microimages of cells. Optoelectron, Instrum Data Process 49(3), 298–304.CrossRefGoogle Scholar
Sinha, A.Kr. & Bhattacharya, D. (2013). Application of nanostructured materials in lasers and display international. J Sci Res Pub 3(5), 1–4.Google Scholar
Sokolov, A.L. (2009). Polarization structure of radiation in the focal region of a lens. Opt Spectrosc 107(2), 207–211.CrossRefGoogle Scholar