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Oligomers Modulate Interfibril Branching and Mass Transport Properties of Collagen Matrices

Published online by Cambridge University Press:  10 July 2013

Catherine F. Whittington ,
Eric Brandner ,
Ka Yaw Teo ,
Bumsoo Han ,
Eric Nauman  and
Sherry L. Voytik-Harbin
Catherine F. Whittington
Affiliation:
Weldon School of Biomedical Engineering, College of Engineering, Purdue University, West Lafayette, IN 47907, USA
Eric Brandner
Affiliation:
Weldon School of Biomedical Engineering, College of Engineering, Purdue University, West Lafayette, IN 47907, USA
Ka Yaw Teo
Affiliation:
School of Mechanical Engineering, College of Engineering, Purdue University, West Lafayette, IN 47907, USA
Bumsoo Han
Affiliation:
Weldon School of Biomedical Engineering, College of Engineering, Purdue University, West Lafayette, IN 47907, USA School of Mechanical Engineering, College of Engineering, Purdue University, West Lafayette, IN 47907, USA
Eric Nauman
Affiliation:
Weldon School of Biomedical Engineering, College of Engineering, Purdue University, West Lafayette, IN 47907, USA School of Mechanical Engineering, College of Engineering, Purdue University, West Lafayette, IN 47907, USA Department of Basic Medical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA
Sherry L. Voytik-Harbin*
Affiliation:
Weldon School of Biomedical Engineering, College of Engineering, Purdue University, West Lafayette, IN 47907, USA Department of Basic Medical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA
*
*Corresponding author. E-mail: harbins@purdue.edu
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Abstract

Mass transport within collagen-based matrices is critical to tissue development, repair, and pathogenesis, as well as the design of next-generation tissue engineering strategies. This work shows how collagen precursors, specified by intermolecular cross-link composition, provide independent control of collagen matrix mechanical and transport properties. Collagen matrices were prepared from tissue-extracted monomers or oligomers. Viscoelastic behavior was measured in oscillatory shear and unconfined compression. Matrix permeability and diffusivity were measured using gravity-driven permeametry and integrated optical imaging, respectively. Both collagen types showed an increase in stiffness and permeability hindrance with increasing collagen concentration (fibril density); however, different physical property–concentration relationships were noted. Diffusivity was not affected by concentration for either collagen type over the range tested. In general, oligomer matrices exhibited a substantial increase in stiffness and only a modest decrease in transport properties when compared with monomer matrices prepared at the same concentration. The observed differences in viscoelastic and transport properties were largely attributed to increased levels of interfibril branching within oligomer matrices. The ability to relate physical properties to relevant microstructure parameters, including fibril density and interfibril branching, is expected to advance the understanding of cell–matrix signaling, as well as facilitate model-based prediction and design of matrix-based therapeutic strategies.

Keywords

collagen matrixoligomermonomermass transportpermeabilitydiffusivity
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 5 , October 2013 , pp. 1323 - 1333
Copyright
Copyright © Microscopy Society of America 2013 

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Whittington Supplementary Material

Supplementary Movie 1. Confocal image stacks for monomer (A, B) and oligomer (C, D) matrices (1.5 mg/mL) collected in combined immunofluorescence and backscattered (reflected) light. Animations show image slice collection (XY plane) at 0.10 um increments through a thickness (A, C) and rotations of reconstructed 3D images (B, D).

Download Whittington Supplementary Material(Video)
Video 1.2 MB

Whittington Supplementary Material

Supplementary Movie 1. Confocal image stacks for monomer (A, B) and oligomer (C, D) matrices (1.5 mg/mL) collected in combined immunofluorescence and backscattered (reflected) light. Animations show image slice collection (XY plane) at 0.10 um increments through a thickness (A, C) and rotations of reconstructed 3D images (B, D).

Download Whittington Supplementary Material(Video)
Video 7.9 MB

Whittington Supplementary Material

Supplementary Movie 1. Confocal image stacks for monomer (A, B) and oligomer (C, D) matrices (1.5 mg/mL) collected in combined immunofluorescence and backscattered (reflected) light. Animations show image slice collection (XY plane) at 0.10 um increments through a thickness (A, C) and rotations of reconstructed 3D images (B, D).

Download Whittington Supplementary Material(Video)
Video 1.4 MB

Whittington Supplementary Material

Supplementary Movie 1. Confocal image stacks for monomer (A, B) and oligomer (C, D) matrices (1.5 mg/mL) collected in combined immunofluorescence and backscattered (reflected) light. Animations show image slice collection (XY plane) at 0.10 um increments through a thickness (A, C) and rotations of reconstructed 3D images (B, D).

Download Whittington Supplementary Material(Video)
Video 7.5 MB