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Practical Methods for TEM, Part II.

Published online by Cambridge University Press:  02 July 2020

K. Chien ,
R. Gonzalez ,
R.C. Heusser ,
H. Shiroishi  and
M.L. Heathershaw
K. Chien
Affiliation:
Pathology & Laboratory Medicine, Cedars-Sinai medical Center, Los Angeles, CA90048
R. Gonzalez
Affiliation:
Pathology & Laboratory Medicine, Cedars-Sinai medical Center, Los Angeles, CA90048
R.C. Heusser
Affiliation:
Pathology & Laboratory Medicine, Cedars-Sinai medical Center, Los Angeles, CA90048
H. Shiroishi
Affiliation:
Pathology & Laboratory Medicine, Cedars-Sinai medical Center, Los Angeles, CA90048
M.L. Heathershaw
Affiliation:
Pathology & Laboratory Medicine, Cedars-Sinai medical Center, Los Angeles, CA90048
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Extract

This is an extension of our presentation from last year. We intend to continue the discussion and review several practical procedures developed in our laboratory for routine and immuno-electron microscopy.

TEM Vs IM-LM In the last decade, the use of diagnostic TEM in the medical field has experienced a drastic decline. The main reason is due to the advances of immuno-labeling techniques for light microscopy and also the decreased coverage of medical insurance for EM. Currently, immuno-histochemistry or in situ hybridization staining can be performed on large paraffin or frozen sections. These procedures can be performed quickly and much less expensively than TEM. However, TEM is still needed when PAP staining is not specific or minimum numbers of cells are stained. It is also possible to process immuno-labeled paraffin slides for TEM examination to confirm a diagnosis. In addition, when only a few microorganisms such as pneumocystic carinii or protothecosis are stained by Grocott’s methenamine silver in paraffin sections, a re-examination and confirmation by TEM can be performed without the use of uranium or lead staining.

Type
Technologists’ Forum: Special Topics and Symposium
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 337 - 338
Copyright
Copyright © Microscopy Society of America 1997

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References

references

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