Hostname: page-component-848d4c4894-4hhp2 Total loading time: 0 Render date: 2024-06-09T08:39:25.461Z Has data issue: false hasContentIssue false
  • English
  • Français

Quantitative 3d Analysis Of Intra-Nuclear Organization In The Tissue Context

Published online by Cambridge University Press:  02 July 2020

S.J. Lockett ,
D.W. Knowles ,
D. Pinkel  and
C. Ortiz de Solórzano
S.J. Lockett
Affiliation:
Life Sciences Division, Ernest Orlando Lawrence Berkeley National Laboratory, CA94720
D.W. Knowles
Affiliation:
Life Sciences Division, Ernest Orlando Lawrence Berkeley National Laboratory, CA94720
D. Pinkel
Affiliation:
UCSF Cancer Center, 2340 Sutter St, San Francisco, CA94143
C. Ortiz de Solórzano
Affiliation:
Life Sciences Division, Ernest Orlando Lawrence Berkeley National Laboratory, CA94720
Get access

Extract

Confocal microscopy is revealing associations between the internal organization of the nucleus and tissue architecture and function. Such associations may exist which are too subtle or complex for visual observation or quantitative analysis may be required, for example as input data to mathematical modeling of cellular processes. In these circumstances, it is necessary to perform the analysis using computer algorithms. We have developed 3D image analysis (IA) algorithms for segmenting nuclei from within intact tissue specimens, measuring the structure of the nuclei and for segmenting specifically- labeled punctate entities within nuclei. In this study we developed algorithms for measuring the spatial organization of the two copies of a specific DNA locus (labeled using fluorescence in situ hybridization (FISH) ) inside diploid nuclei and with respect to the nuclear organization of the tissue.

For each segmented nucleus, its center of mass (CoM) was determined, which informed us about its position in the tissue.

Type
Cytochemistry
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1320 - 1321
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Höfers>, C.> et al, Bioimaging 1(1993)96.3.3.CO;2-4>CrossRef,+C.>+et+al,+Bioimaging1(1993)96.>Google Scholar
2.Leliève, S. A. et al., Proc Natl Acad Sci 95(1998)14711.CrossRefGoogle Scholar
3.Lo, C. H. and Don., H. S.IEEE Trans Patt Anal Mach Intell 11(1989)1053.CrossRefGoogle Scholar
4.Ortiz de Solórzano, C. et al., J Micros 193(1999)212.CrossRefGoogle Scholar
5.Ortiz de Solórzano, C. et al., this meeting, submitted.Google Scholar
6.Thompson, C. T. et al., Am J Pathol 144(1994)237.Google Scholar
7.Supported by the Director, Office of Energy Research, Office of Health and Environmental Research of the U.S. Department of Energy under contract NO. DE-AC03-76SF00098, NIH grant CA-67412 and a contract with Carl Zeiss Inc.Google Scholar