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Quantitative Three-Dimensional Image Analysis: Confocal and Widefield Light Microscopy

Published online by Cambridge University Press:  02 July 2020

B. Roysam ,
T. J. Holmes ,
D. H. Szarowski  and
J. N. Turner
B. Roysam
Affiliation:
Rensselaer Polytechnic Institute, and AutoQuant Imaging, Troy, NY, 12180
T. J. Holmes
Affiliation:
Rensselaer Polytechnic Institute, and AutoQuant Imaging, Troy, NY, 12180
D. H. Szarowski
Affiliation:
Wadsworth Center, New York State Department of Health, Albany, NY, 12201
J. N. Turner
Affiliation:
Wadsworth Center, New York State Department of Health, Albany, NY, 12201
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Three-dimensional (3-D) light microscopy has been rapidly expanding for several years, both with respect to instrumentation, and biological applications. This new technology has transformed light microscopy from two-dimensional imaging of 3-D objects to a fully 3-D science. To date, qualitative analysis, reconstruction, and visualization has received far more attention compared to quantitative analysis. The motivations for 3-D image analysis are manifold, and include more accurate morphometry, separation of overlapping objects, and analysis of non-planar regions of interest and of thick specimens without the artifacts arising from physical sectioning. Furthermore, automating this analysis makes it fast, inexpensive, objective, and precise, and the results can be validated by manually confirming the analysis for any selected subset of 3-D images.

Increasingly, quantitative 3-D image analysis is emerging as an attractive technology for many applications. Studies include the testing of human-use products (shampoos, laxatives etc.), drug development, aging and development, cell loss/proliferation, fetal alcohol syndrome, and analysis of medical samples such as Pap smears, breast biopsies and a variety of cancers.

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Tutorials
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 377 - 378
Copyright
Copyright © Microscopy Society of America 1997

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