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    Moreau, Jean-David Gomez, Bernard Daviero-Gomez, Véronique Néraudeau, Didier and Tafforeau, Paul 2016. Inflorescences of Mauldinia sp. (Lauraceae) and associated fruits from the Cenomanian of Languedoc Roussillon, France. Cretaceous Research, Vol. 59, p. 18.

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    Glasspool, Ian J. and Scott, Andrew C. 2013. Fire Phenomena and the Earth System.

    Spencer, Alan R.T. Hilton, Jason and Sutton, Mark D. 2013. Combined methodologies for three-dimensional reconstruction of fossil plants preserved in siderite nodules: Stephanospermum braidwoodensis nov. sp. (Medullosales) from the Mazon Creek lagerstätte. Review of Palaeobotany and Palynology, Vol. 188, p. 1.

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    Abel, R.L. Parfitt, S. Ashton, N. Lewis, Simon G. Scott, Beccy and Stringer, C. 2011. Digital preservation and dissemination of ancient lithic technology with modern micro-CT. Computers & Graphics, Vol. 35, Issue. 4, p. 878.

    Scott, Andrew C. 2010. Charcoal recognition, taphonomy and uses in palaeoenvironmental analysis. Palaeogeography, Palaeoclimatology, Palaeoecology, Vol. 291, Issue. 1-2, p. 11.

    Scott, Andrew C. and Damblon, Freddy 2010. Charcoal: Taphonomy and significance in geology, botany and archaeology. Palaeogeography, Palaeoclimatology, Palaeoecology, Vol. 291, Issue. 1-2, p. 1.

    Smith, S. Y. Collinson, M. E. Rudall, P. J. Simpson, D. A. Marone, F. and Stampanoni, M. 2009. Virtual taphonomy using synchrotron tomographic microscopy reveals cryptic features and internal structure of modern and fossil plants. Proceedings of the National Academy of Sciences, Vol. 106, Issue. 29, p. 12013.


Scanning Electron Microscopy and Synchrotron Radiation X-Ray Tomographic Microscopy of 330 Million Year Old Charcoalified Seed Fern Fertile Organs

  • Andrew C. Scott (a1), Jean Galtier (a2), Neil J. Gostling (a3) (a4), Selena Y. Smith (a1), Margaret E. Collinson (a1), Marco Stampanoni (a5) (a6), Federica Marone (a5), Philip C.J. Donoghue (a3) and Stefan Bengtson (a7)
  • DOI:
  • Published online: 01 March 2009

Abundant charcoalified seed fern (pteridosperm) pollen organs and ovules have been recovered from Late Viséan (Mississippian 330 Ma) limestones from Kingswood, Fife, Scotland. To overcome limitations of data collection from these tiny, sometimes unique, fossils, we have combined low vacuum scanning electron microscopy on uncoated specimens with backscatter detector and synchrotron radiation X-ray tomographic microscopy utilizing the Materials Science and TOMCAT beamlines at the Swiss Light Source of the Paul Scherrer Institut. In combination these techniques improve upon traditional cellulose acetate peel sectioning because they enable study of external morphology and internal anatomy in multiple planes of section on a single specimen that is retained intact. The pollen organ Melissiotheca shows a basal parenchymatous cushion bearing more than 100 sporangia on the distal face. Digital sections show the occurrence of pollen in some sporangia. The described ovule is new and has eight integumentary lobes that are covered in spirally arranged glandular hairs. Virtual longitudinal sections reveal the lobes are free above the pollen chamber. Results are applied in taxonomy and will subsequently contribute to our understanding of the former diversity and evolution of ovules, seeds, and pollen organs in the seed ferns, the first seed-bearing plants to conquer the land.

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M.I. Bird , P.L Ascough , I.M. Young , C.V. Wood & A.C. Scott (2008). X-ray microtomographic imaging of charcoal. J Archaeol Sci 35, 26982706 (doi:10.1016/j.jas.2008.04.018).

P.C.J. Donoghue , S. Bengtson , X-P. Dong , N.J. Gostling , T. Huldtgren , J.A. Cunningham , C. Yin , Z. Yue , F. Peng & M. Stampanoni (2006). Synchrotron X-ray tomographic microscopy of fossil embryos. Nature 442, 680683 (doi:10.1038/nature04890).

E.M. Friis , P.R. Crane , K.P. Pedersen , S. Bengtson , P.C.J. Donoghue , G.W. Grimm & M. Stampanoni (2007). Phase-contrast X-ray microtomography links Cretaceous seeds with Gnetales and Bennettitales. Nature 450, 549552.

I.J. Glasspool , D. Edwards & L. Axe (2004). Charcoal in the Silurian as evidence for the earliest wildfire. Geology 32, 381383.

I.J. Glasspool , D. Edwards & L. Axe (2006). Charcoal in the Early Devonian: A wildfire-derived Konservat-Lagerstatte. Rev Palaeobot Palynol 142, 131136.

J. Hilton & R.B. Bateman (2006). Pteridosperms are the backbone of seed-plant phylogeny. J Torrey Bot Soc 133, 119168.

R. Lupia (1995). Paleobotanical data from fossil charcoal—An actualistic study of plant reproductive structures. Palaios 10, 465477.

B. Meyer-Berthaud (1986). Melissiotheca: A new pteridosperm pollen organ from the Lower Carboniferous of Scotland. Bot J Linn Soc 93, 277290.

B. Meyer-Berthaud & J. Galtier (1986). Studies on a Lower Carboniferous flora from Kingswood near Pettycur, Scotland. II. Phacelotheca, a new synangiate fructification of pteridospermous affinities. Rev Palaeobot Palynol 48, 181198.

M.A. Millay & T.N. Taylor (1979). Paleozoic seed fern pollen organs. Bot Rev 45, 301375.

K.J. Niklas , B.H. Tiffney & A.H. Knoll (1983). Patterns in vascular land plant diversification. Nature 303, 614616.

J. Schönenberger (2005). Rise from the ashes—The reconstruction of charcoal fossil flowers. Trends Plant Sci 10, 436443.

A.C. Scott (1990b). Preservation, evolution and extinction of plants in Lower Carboniferous volcanic sequences in Scotland. In Volcanism and Fossil Biotas. Geological Society of America Special Publication, M. Lockley & A. Rice (Eds.), vol. 244, pp. 2538.

A.C. Scott (2000). The pre-Quaternary history of fire. Palaeogeog Palaeoclimatol Palaeoecol 164, 281329.

A.C. Scott & M.E. Collinson (2003). Non-destructive multiple approaches to interpret the preservation of plant fossils: Implications for calcium-rich permineralisations. J Geol Soc Lond 160, 857862.

A.C. Scott , J. Galtier & G. Clayton (1985). A new late Tournaisian (Lower Carboniferous) flora from the Kilpatrick Hills, Scotland. Rev Palaeobot Palynol 44, 8199.

A.C. Scott & I.J. Glasspool (2006). The diversification of Paleozoic fire systems and fluctuations in atmospheric oxygen concentration. Proc Natl Acad Sci USA 103, 1086110865.

A.C. Scott , B. Meyer-Berthaud , J. Galtier , G.M. Rex , S.A. Brindley & G. Clayton (1986). Studies on a Lower Carboniferous flora from Kingswood near Pettycur, Scotland. I. Preliminary report. Rev Palaeobot Palynol 48, 161180.

R. Serbet , T.N. Taylor & E.L. Taylor (2006). On a new medullosan pollen organ from the Pennsylvanian of North America. Rev Palaeobot Palynol 142, 219227.

M. Stampanoni , G.L. Borchert , P. Wyss , R. Abela , B.D. Patterson , S. Hunt , D. Vermeulen & P. Rüegsegger (2002). High resolution X-ray detector for synchrotron-based microtomography. Nucl Instrum Meth Phys Res A 491, 291301.

M.D. Sutton (2008). Tomographic techniques for the study of exceptionally preserved fossils. Proc Roy Soc B 275, 15871593 (doi: 10.1098/rspb.2008.0263).

P. Tafforeau , R. Boistel , E. Boller , A. Bravin , M. Brunet , Y. Chaimanee , P. Cloetens , M. Feist , J. Hoszowska , J.-J. Jaeger , R.F. Kay , V. Lazzari , L. Marivaux , A. Nel , C. Nemoz , X. Thibault , P. Vignaud & S. Zabler (2006). Applications of X-ray synchrotron microtomography for non-destructive 3D studies of paleontological specimens. Appl Phys A 83, 195202.

M. Von Balthazar , K.R. Pedersen , P.R. Crane , M. Stampanoni & E.M. Friis (2007). Potomacanthus lobatus gen. et sp nov., a new flower of probable Lauraceae from the Early Cretaceous (Early to Middle Albian) of eastern North America. Am J Bot 94, 20412053.

T. Weitkamp , C. Raven & A. Snigirev (1999). An imaging and microtomography facility at the ESRF beamline ID 22. In Developments in X-Ray Tomography II, Bonse, U. (Ed.), Proc. SPIE 3772, 311.

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