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Staining Characteristics of Lonchocarpus cyanescens Leaf Extract on the Testis of Sprague-Dawley Rats

Published online by Cambridge University Press:  26 July 2012

Rosemary B. Bassey ,
Airat A. Bakare ,
Innocent A. Edagha ,
Abraham A.A. Osinubi  and
Ademola A. Oremosu
Rosemary B. Bassey*
Affiliation:
Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo, Nigeria
Airat A. Bakare
Affiliation:
Department of Anatomy, College of Medicine, University of Lagos, Lagos, Nigeria
Innocent A. Edagha
Affiliation:
Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo, Nigeria
Abraham A.A. Osinubi
Affiliation:
Department of Anatomy, College of Medicine, University of Lagos, Lagos, Nigeria
Ademola A. Oremosu
Affiliation:
Department of Anatomy, College of Medicine, University of Lagos, Lagos, Nigeria
*
Corresponding author. E-mail: rosemary_bassey@yahoo.com
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Abstract

The use of nonallergic, nontoxic, and eco-friendly natural dyes has become a matter of significant importance due to increased environmental awareness on the need to avoid hazardous synthetic dyes. This study was to determine the staining properties of the dye extract of Lonchocarpus cyanescens on histomorphology of the testis. Freshly cut leaves of L. cyanescens obtained from Akpan Ifia Inan village in Ikono local government area of Akwa Ibom state (latitude 5° 10′ 12″ N; longitude 7° 48′ 0″ E) were put into a plastic jar and boiling water was poured to cover the leaves. It was covered and left for an hour. The liquid was strained and potassium hydroxide was added to the dye water mixture to reach a pH of 9. A whisk was used to mix air into the liquid, and the mixuture was then allowed to sit until the blue indigo had settled to the bottom of the container. The dye was diluted with 70% ethanol to a concentration of 0.1 g/mL and was used to stain sections of testes. Its potential for use as a counterstain was also investigated. The testes sections were stained in shades of blue. The dye overshadowed the colors of haematoxylin and eosin. Preliminary phytochemical screening of L. cyanescens revealed that it contains alkaloids, saponins, flavonoids, and tannins.

Keywords

Lonchocarpus cyanescensindigodyestainstestis
Type
Biological Applications: Techniques, Software, and Equipment Development
Information
Microscopy and Microanalysis , Volume 18 , Issue 4 , August 2012 , pp. 840 - 843
Copyright
Copyright © Microscopy Society of America 2012

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