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Stereological Analysis of Mitochondria and Smooth Endoplasmic Reticulum Distribution in Human Oocytes at Prophase I

Published online by Cambridge University Press:  03 October 2008

A. Luís ,
E. Rocha ,
E Oliveira ,
J. Silva ,
A. Barros  and
M. Sousa
A. Luís
Affiliation:
Lab Cell Biology, ICBAS, University of Porto, Largo Prof. Abel Salazar 2, 4099-001 Porto, Portugal
E. Rocha
Affiliation:
Lab Cell Biology, ICBAS, University of Porto, Largo Prof. Abel Salazar 2, 4099-001 Porto, Portugal
E Oliveira
Affiliation:
Lab Cell Biology, ICBAS, University of Porto, Largo Prof. Abel Salazar 2, 4099-001 Porto, Portugal
J. Silva
Affiliation:
Centre for Reproductive Genetics A. Barros, Avenida do Bessa 591, 1º dto frente, 4100-009 Porto, Portugal
A. Barros
Affiliation:
Centre for Reproductive Genetics A. Barros, Avenida do Bessa 591, 1º dto frente, 4100-009 Porto, Portugal Dept. Genetics, Fac. Medicine, University of Porto, Alameda Prof. Hernâni Monteiro 4200 - 319 Porto, Portugal
M. Sousa
Affiliation:
Lab Cell Biology, ICBAS, University of Porto, Largo Prof. Abel Salazar 2, 4099-001 Porto, Portugal

Abstract

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The structure of human oocytes at the different maturation stages is known based on descriptive studies of its ultrastructural composition. The tridimensional quantitative ultrastructural analysis of human oocytes would be important for understanding folliculogenesis and establishing adequate methods of in vitro oocyte maturation. In this study, stereological methods were applied to quantify the distribution of mitochondria (M) and smooth endoplasmic reticulum (SER) in oocytes at the germinal vesicle (VG) stage of prophase I.

Type
Abstract
Information
Microscopy and Microanalysis , Volume 14 , Supplement S3 , September 2008 , pp. 103 - 104
Copyright
Copyright © Microscopy Society of America 2008