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Subdaily Growth Patterns and Organo-Mineral Nanostructure of the Growth Layers in the Calcitic Prisms of the Shell of Concholepas concholepas Bruguière, 1789 (Gastropoda, Muricidae)

  • Nury Guzman (a1), Alexander D. Ball (a2), Jean-Pierre Cuif (a3), Yannicke Dauphin (a3), Alain Denis (a3) and Luc Ortlieb (a1)...

Fluorochrome marking of the gastropod Concholepas concholepas has shown that the prismatic units of the shell are built by superimposition of isochronic growth layers of about 2 μm. Fluorescent growth marks make it possible to establish the high periodicity of the cyclic biomineralization process at a standard growth rhythm of about 45 layers a day. Sulphated polysaccharides have been identified within the growth layers by using synchrotron radiation, whereas high resolution mapping enables the banding pattern of the mineral phase to be correlated with the layered distribution of polysaccharides. Atomic force microscopy has shown that the layers are made of nanograins densely packed in an organic component.

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Addadi, L., Joester, D., Nudelman, F. & Weiner, S. (2006). Mollusk shell formation: A source of new concepts for understanding biomineralization processes. Chemistry 12, 980987.
Adkins, J.F., Boyle, E.A., Curry, W.B. & Lutringer, A. (2003). Stable isotopes in deep-sea corals and a new mechanism for “vital effects.” Geochim Cosmochim Acta 67, 11291143.
Blamart, B., Rollion-Bard, C., Cuif, J.P., Juillet-Leclerc, A., Lutringer, A. & Van Weering, T.C.E. (2005). C and O isotopes in a deep-sea coral (Lophelia pertusa) related to a skeletal microstructure. In Cold-Water Corals and Ecosystems, Freiwald, A. & Roberts, J.M. (Eds.), pp. 10051020. Berlin, Heidelberg: Springer Verlag.
Clarke, A., Prothero-Thomas, E., Beaumont, J.C., Chapman, A.L. & Brey, T. (2004). Growth in the limpet Nacella concinna from contrasting sites in Antarctica. Polar Biol 28, 6271.
Cuif, J.P., Dauphin, Y., Doucet, J., Salomé, M. & Susini, J. (2003). XANES mapping of organic sulfate in three scleractinian coral skeletons. Geochim Cosmochim Acta 67, 7583.
Dauphin, Y., Cuif, J.P., Doucet, J., Salomé, M., Susini, J. & Williams, C.T. (2003a). In situ chemical speciation of sulfur in calcitic biominerals and the simple prism concept. J Struct Biol 142, 272280.
Dauphin, Y., Cuif, J.P., Doucet, J., Salomé, M., Susini, J. & Williams, C.T. (2003b). In situ mapping of growth lines in the calcitic prismatic layers of mollusc shells using X-ray absorption near-edge structure (XANES) spectroscopy at the sulphur K-edge. Mar Biol 142, 299304.
Dauphin, Y., Cuif, J.P., Salomé, M., Susini, J. & Williams, C.T. (2006). Microstructures and chemical composition of giant avian eggshells. Anal Bioanal Chem 386, 761771.
Dauphin, Y., Guzman, N., Denis, A., Cuif, J.P. & Ortlieb, L. (2003c). Microstructure, nanostructure and composition of the shell of Concholepas concholepas (Gastropoda, Muricidae). Aquat Liv Res 16, 95103.
Frémy, M. (1855). Recherches chimiques sur l'os. Ann Chem Phys 43, 47107.
Guzman, N. (2004). Validation d'une approche scléroclimatologique sur la côte du Chili et du Pérou par l'analyse microstructurale et biogéochimique des coquilles du gastéropode Concholepas concholepas (Bruguière, 1789). Thèse Université Paris XI, 219 p.
Heilmayer, O., Honnen, C., Jacob, U., Chiantore, M., Cattaneao-Vietti, R. & Brey, T. (2005). Temperature effects on summer growth rates in the antarctic scallop, Adamussium colbeicki. Polar Biol 2, 523527.
Hidi, H. & Hanks, J.E. (1968). Vital staining of bivalve mollusk shells with alizarin sodium monosulfonite. Proc Nation Shellfish Assoc 53, 3741.
Iyengar, E.V. (2002). Sneaky snails and wasted worms: Kleptoparasitism by Trichotropis cancellata (Mollusca, Gastropoda) on Serpula columbiana (Annelida, Polychaeta). Mar Ecol Prog Ser 244, 153162.
Kaehler, S. & McQuaid, C.D. (1999). Use of fluorochrome calcein as an in situ growth marker in the brown mussel Perna perna. Mar Biol 133, 455460.
Koike, H. (1973). Daily growth lines of the clam, Meretrix lusoria—A basic study for the estimation of prehistoric seasonal gathering. J Anthrop Soc Nippon 81, 122138.
Meibom, A., Cuif, J.P., Hillion, F., Constantz, B., Juillet-Leclerc, A., Dauphin, Y., Watanabe, T. & Dunbar, R.B. (2004). Distribution of magnesium in coral skeleton. Geophys Res Lett 31, L23306 doi 10.1029/2044GL021313.
Mohler, J.W. (1997). Immersion of larval Atlantic salmon in calcein solutions to induce a non-lethally detectable mark. N Am J Fish Manag 17, 751756.
Moran, A.L. (2000). Calcein as a marker in experimental studies on newly-hatched gastropods. Mar Biol 137, 893898.
Rollion-Bard, C., Blamart, D., Cuif, J.P. & Juillet-Leclerc, A. (2003). Microanalysis of C and O isotopes of azooxanthellate and zooxanthellate corals by ion microprobe. Coral Reefs 22, 405415.
Urey, H.C., Lowenstam, H.A., Epstein, S. & McKinney, C.R. (1951). Measurement of palaeotemperatures and temperatures of the Upper Cretaceous of England, Denmark, and the Southeastern United States. Bull Geol Soc Am 82, 399416.
Vermeij, G.J. & Carlson, S.J. (2000). The muricid gastropod subfamily Rapaninae: Phylogeny and ecological history. Paleobiology 26, 1946.
Weiner, S. & Dove, P.M. (2003). An overview of biomineralization processes and the problem of the vital effect. In Biomineralization, Dove, P.M., DeYoreo, J.J. & Weiner, S. (Eds.), S54, pp. 129.
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Microscopy and Microanalysis
  • ISSN: 1431-9276
  • EISSN: 1435-8115
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