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Three Dimensional Imaging of Biological Samples and Nano-materials Using Soft X-ray Microscopy

Published online by Cambridge University Press:  10 August 2018

Gang Liu ,
Liang Chen  and
Yong Guan
Gang Liu*
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.
Liang Chen
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.
Yong Guan
Affiliation:
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.
*
*Gang Liu, liugang@ustc.edu.cn

Abstract

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The soft X-ray Microscopy beamline BL07W at National Synchrotron Radiation Laboratory is devoted to cryo nano-tomography for biological applications in the water window (284 - 530 eV) and for imaging of nanomaterials from 200 to 2500 eV. An ellipsoidal capillary used as condense to focus monochromatic light onto the sample. Two Ni zone plate (ZP) lenses made by Zeiss with 40 nm and 25 nm outer most zone widths, respectively, are available, giving spatial resolution in 2D of down to 40 nm and 30 nm, respectively. Hydrated biological specimens had been imaged in the water window photon energy range without chemical fixation, dehydration, chemical staining and physical sectioning. In addition, other applications such as nanomaterials imaging had been demonstrated.

Keywords

Soft X-ray Microscopywater windowcryo nano-tomographynanomaterials imaging Introduction:
Type
Abstract
Information
Microscopy and Microanalysis , Volume 24 , Supplement S2: Proceedings of the 14th International Conference on X-ray Microscopy (XRM2018) , August 2018 , pp. 392 - 393
Copyright
© Microscopy Society of America 2018 

References

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