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Three Dimensional Imaging of Biological Samples and Nano-materials Using Soft X-ray Microscopy

  • Gang Liu (a1), Liang Chen (a1) and Yong Guan (a1)
Abstract

The soft X-ray Microscopy beamline BL07W at National Synchrotron Radiation Laboratory is devoted to cryo nano-tomography for biological applications in the water window (284 - 530 eV) and for imaging of nanomaterials from 200 to 2500 eV. An ellipsoidal capillary used as condense to focus monochromatic light onto the sample. Two Ni zone plate (ZP) lenses made by Zeiss with 40 nm and 25 nm outer most zone widths, respectively, are available, giving spatial resolution in 2D of down to 40 nm and 30 nm, respectively. Hydrated biological specimens had been imaged in the water window photon energy range without chemical fixation, dehydration, chemical staining and physical sectioning. In addition, other applications such as nanomaterials imaging had been demonstrated.

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Copyright
Corresponding author
*Gang Liu, liugang@ustc.edu.cn
References
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[1] Chichon, FJ, et al, Journal of Structural Biology 177 2012 202211.
[2] Drescher, D, et al, Nanoscale 5(19 2013 91939198.
[3] Liu, J, et al, Journal of Microscopy 270 2018 6470.
[4] Ma, T, et al, Advanced materials 1706435 2018 18. doi: 10.1002/adma.201706435.
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Microscopy and Microanalysis
  • ISSN: 1431-9276
  • EISSN: 1435-8115
  • URL: /core/journals/microscopy-and-microanalysis
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