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Reconstructing What Was: Software Applied To Serial Section TEM

Published online by Cambridge University Press:  14 March 2018

Marcia D. Feinberg  and
John C. Fiala
Marcia D. Feinberg*
Affiliation:
Boston University, Boston MA
John C. Fiala
Affiliation:
Boston University, Boston MA
*
mfein@bu.edu

Extract

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The well-known recipe for serial reconstruction in microscopy consists of the following six steps: fix and embed specimen, cut and mount sections for microscopy, image sections, align images in the computer, identify and trace profiles in images, generate three-dimensional (3D) visualizations. The free software program Reconstruct facilitates the final steps of this process for many types of microscopy, including scanning electron or ion beam microscopy applied to material and light microscopy applied to biological samples. We describe here how serial sectioning and transmission electron microscopy (TEM) can be combined with the Reconstruct software to generate large volumes of soft tissue ultrastructure for analysis.

Type
Research Article
Information
Microscopy Today , Volume 15 , Issue 5 , September 2007 , pp. 26 - 31
Copyright
Copyright © Microscopy Society of America 2007

References

1 Download sites: http://synapses.bu.edu/ or http://tech.groups.yahoo.com/group/reconstruct_users/ Google Scholar
2 Bozzola, JJ, Russell, LD (1998) Electron Microscopy. Principles and Techniques for Biologists, Second Edition. Jones and Bartlett Publishers: Boston.Google Scholar
3 Elliott, D (2007) Serial sectioning via microtomy (or, how to get over 100 consecutive sections on one TEM grid). Microscopy Today 15(1):30–33.Google Scholar
4 Fiala, JC (2005) Reconstruct: A free editor for serial section microscopy. J Microscopy 218:52–61.CrossRefGoogle ScholarPubMed
5 Fiala, JC, Feinberg, M, Peters, A, Barbas, H (2007) Mitochondrial degeneration in dystrophic neurites of senile plaques may lead to extracellular deposition of fine filaments. Brain Struct Funct (in press).CrossRefGoogle Scholar
6 Fiala, JC, Feinberg, M, Popov, V, Harris, KM (1998) Synaptogenesis via dendritic filopodia in developing hippocampal area CA1. J Neurosci 18:8900–8911.Google Scholar
7 Giberson, RT, Demaree, RS Jr. (2001) Microwave Techniques and Protocols. Humana Press, Totowa, NJ.Google Scholar
8 Glauert, AM, Lewis, PR (1998) Biological Specimen Preparation for Transmission Electron Microscopy, Princeton University Press, Princeton, New Jersey.Google Scholar
9 Harris, KM, Perry, E, Bourne, J, Feinberg, M, Ostroff, L, Hurlburt, J (2006) Uniform serial sectioning for transmission electron microscopy. J Neurosci 26:12101–12103.Google Scholar
10 Hayat, MA (2000) Principles and Techniques of Electron Microscopy. Biological Applications, 4th edition, Cambridge University Press.Google Scholar
11 Hoffpauir, BK, Pope, BA, Spirou, GA (2007) Serial sectioning and electron microscopy of large tissue volumes for 3D analysis and reconstruction: a case study of the calyx of Held. Nature Protocols 2(1):9–22.Google Scholar