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Restoration of Light Sheet Multi-View Data with the Huygens Fusion and Deconvolution Wizard

  • Peter J. Verveer (a1), Vincent T.G. Schoonderwoert (a1), Denis Ressnikoff (a2), Shane D. Elliott (a3), Kiefer D. van Teutem (a1), Tobias C. Walther (a3) and Hans T.M. van der Voort (a1)...
Abstract:

Light sheet fluorescence microscopy (LSFM) allows for high-resolution three-dimensional imaging with minimal photo-damage. By viewing the sample from different directions, different regions of large specimens can be imaged optimally. Moreover, owing to their good spatial resolution and high signal-to-noise ratio, LSFM data are well suited for image deconvolution. Here we present the Huygens Fusion and Deconvolution Wizard, a unique integrated solution for restoring LSFM images, and show that improvements in signal and resolution of 1.5 times and higher are feasible.

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References
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[1] Huisken, J and Stainier, DY, Development 136(1) (2009) 19631975.
[2] Power, RM and Huisken, J, Nat Methods 14(4) (2017) 360373.
[3] Strobl, F et al., Nat Protoc 12(6) (2017) 11031109.
[4] Huisken, J et al., Science 305(568) 2004) 10071009.
[5] Verveer, PJ et al., Nat Methods 4(4) (2007) 311313.
[6] van der Voort, HTM, Super-Resolution Imaging in Biomedicine, eds. A Diaspro and MA van Zandvoort, CRC Press, Boca Raton, FL, 2016, 99119.
[7]For more information, see Scientific Volume Imaging, “Deconvolutoin algorithms: optimize the Huygens deconvolution results,” https://svi.nl/Deconvolution-algorithms (accessed July 15, 2018).
[8]For more information, see Scientific Volume Imaging, “Distorted PSF due to refractive index mismatch,” https://svi.nl/MismatchDistortsPsf (accessed July 15, 2018).
[9] Chen, BC et. al., Science 346(6208) (2014) 1257998/11257998/12.
[10] North, A, J Cell Biol 172(1) (2006) 918.
[11]See also Scientific Volume Imaging, “Data acquisition pitfalls,” https://svi.nl/AcquisitionPitfalls (accessed July 15, 2018).
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Microscopy Today
  • ISSN: 1551-9295
  • EISSN: 2150-3583
  • URL: /core/journals/microscopy-today
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