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RIMAPS and Varlogram Analysis of Barley Leaf Surfaces

Published online by Cambridge University Press:  14 March 2018

Eduardo A. Favret  and
Néstor O. Fuentes
Eduardo A. Favret*
Affiliation:
Comisiόn Nacional de Energía Atόmica, Buenos Aires, Argentina, Instituto de Tecnología “Prof. Jorge A. Sábato, Universidad National de Gral San Martin. Buenos Aires, Argentina
Néstor O. Fuentes
Affiliation:
Comisiόn Nacional de Energía Atόmica, Buenos Aires, Argentina, Instituto de Tecnología “Prof. Jorge A. Sábato, Universidad National de Gral San Martin. Buenos Aires, Argentina
*
favret@cnea.gov.ar

Extract

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It is a common practice to use microscopic images to describe the differences observed between plant tissues. The images illustrate the taxonomic characteristics of the studied species. In this work we introduce a quantitative method for conducting these analyses utilizing digitized images obtained via scanning electron microscopy (SEM) of barley leaf surfaces. The topography of the leaf surfaces of a narrow-leaf mutant and its wild type mother line was characterized, see figure 1, using the Rotated Image with Maximum Average Power Spectrum (RIMAPS) technique and the Variogram method. Spectra resulting from RIMAPS analysis allow us to identify the specimens and to distinguish between the adaxial or the abaxial side of the leaf. These results are complemented by obtaining the typical scale lengths that characterize the abaxial surfaces of both the mutant and the mother line barley leaves.

Type
Research Article
Information
Microscopy Today , Volume 12 , Issue 5 , September 2004 , pp. 24 - 27
Copyright
Copyright © Microscopy Society of America 2004

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