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Some Hints on Clinical TEM Negative Staining for Viruses

Published online by Cambridge University Press:  14 March 2018

Sara E. Miller
Sara E. Miller*
Affiliation:
Duke University Medical School

Extract

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I would not, repeat *not*, recommend fixing any virus sample before attaching it to the grid. If you are concerned with pathogenicity, you can fix it after allowing it to adhere to the grid, then wash with water and stain, or you can UV irradiate both sides of the grid after staining. For speed in reporting clinical results (we do almost 1000/year), we look at negative stains of potentially infectious material without fixing by keeping a separate specimen holder for "dirty" grids and another for nonpathogenic material such as sections. The grids are then UV irradiated before storage,

Type
Research Article
Information
Microscopy Today , Volume 5 , Issue 10 , December 1997 , pp. 10 - 11
Copyright
Copyright © Microscopy Society of America 1997

References

Hayat, & Miller, , 1990. Negative Staining, McGraw-Hill, Especially the chapter on safetyGoogle Scholar