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In Vitro Osteogenic, Angiogenic, and Inflammatory Effects of Copper in β-Tricalcium Phosphate

Published online by Cambridge University Press:  10 January 2019

Weiguo Han ,
Haley Cummings ,
Murali Krishna Duvuuru ,
Sarah Fleck ,
Sahar Vahabzadeh  and
Sherine F. Elsawa
Weiguo Han
Affiliation:
Molecular, Cellular and Biomedical Sciences Department, University of New Hampshire, Rudman Hall, 46 College Road, Durham, NH03824
Haley Cummings
Affiliation:
Department of Mechanical Engineering, Northern Illinois University, 590 Garden Road, DeKalb, IL60115
Murali Krishna Duvuuru
Affiliation:
Department of Mechanical Engineering, Northern Illinois University, 590 Garden Road, DeKalb, IL60115
Sarah Fleck
Affiliation:
Department of Mechanical Engineering, Northern Illinois University, 590 Garden Road, DeKalb, IL60115
Sahar Vahabzadeh*
Affiliation:
Department of Mechanical Engineering, Northern Illinois University, 590 Garden Road, DeKalb, IL60115
Sherine F. Elsawa
Affiliation:
Molecular, Cellular and Biomedical Sciences Department, University of New Hampshire, Rudman Hall, 46 College Road, Durham, NH03824
*
*(Email: svahabzadeh@niu.edu)
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Abstract

Tricalcium phosphate (TCP) is a promising candidate in bone and dental tissue engineering applications. Though osteoconductive, its low osteoinductivity is a major concern. Trace elements addition at low concentrations are known for their impact on not only the osteoinductivity, but also physical and mechanical properties of TCP. Copper (Cu) is known for its role in vascularization and angiogenesis in biological systems. Here, we studied the effects of Cu addition on phase composition, porosity, microstructure and in vitro interaction with osteoblast (OB) cells. Our results showed that Cu stabilized the TCP structure, while no significant effect of microstructure and porosity was found. Cu at concentrations less than 1 wt.% did not have any cytotoxic effect while decreased proliferation of OBs were observed at 1 wt.% Cu doped TCP. Addition of Cu upregulated collagen type I and vascular endothelial growth factor expression in a dose dependent manner at early time-point. Furthermore, Cu reduced inflammatory gene expression by human osteoblasts. These findings show that addition of Cu to TCP may provide a therapeutic strategy that can be applied in bone tissue engineering applications.

Keywords

biomaterialboneCuceramic
Type
Articles
Information
MRS Advances , Volume 4 , Issue 21: Biomaterials and Soft Materials , 2019 , pp. 1253 - 1259
Copyright
Copyright © Materials Research Society 2019 

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