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Physical characterization and in vitro evaluation of 3D printed hydroxyapatite, tricalcium phosphate, zirconia, alumina, and SiAlON structures made by lithographic ceramic manufacturing

Published online by Cambridge University Press:  29 April 2020

Alexander K. Nguyen Open the ORCID record for Alexander K. Nguyen [Opens in a new window] ,
Peter L. Goering ,
Shelby A. Skoog  and
Roger J. Narayan
Alexander K. Nguyen
Affiliation:
Joint UNC/NCSU Department of Biomedical Engineering, North Carolina State University, Raleigh, North Carolina, United States Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD
Peter L. Goering
Affiliation:
Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD
Shelby A. Skoog
Affiliation:
Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD
Roger J. Narayan*
Affiliation:
Joint UNC/NCSU Department of Biomedical Engineering, North Carolina State University, Raleigh, North Carolina, United States
*
*(Email: RogerNarayan@scholarone.com)
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Abstract

In this study, lithographic ceramic manufacturing was used to create solid chips out of hydroxyapatite, tricalcium phosphate, zirconia, alumina, and SiAlON ceramic. X-ray powder diffraction of each material confirmed that the chips were crystalline, with little amorphous character that could result from remaining polymeric binder, and were composed entirely out of the ceramic feedstock. Surface morphologies and roughnesses were characterized using atomic force microscopy. Human bone marrow stem cells cultured with osteogenic supplements on each material type expressed alkaline phosphatase levels, an early marker of osteogenic differentiation, on par with cells cultured on a glass control. However, cells cultured on the tricalcium phosphate-containing material expressed lower levels of ALP suggesting that osteoinduction was impaired on this material. Further analyses should be conducted with these materials to identify underlying issues of the combination of material and analysis method.

Keywords

biomaterialadditive manufacturing
Type
Articles
Information
MRS Advances , Volume 5 , Issue 46-47: Soft Materials and Biomaterials , 2020 , pp. 2419 - 2428
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Copyright
Copyright © Materials Research Society 2020

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References

References:

Schwentenwein, M. , Schneider, P. , and Homa, J. , Lithography-Based Ceramic Manufacturing: A Novel Technique for Additive Manufacturing of High-Performance Ceramics. Advances in Science and Technology, 2014. 88: p. 60-64.CrossRefGoogle Scholar
Chen, T.-H. , et al., Lattice Microarchitecture for Bone Tissue Engineering from Calcium Phosphate Compared to Titanium. Tissue Engineering Part A, 2018. 24(19-20): p. 1554-1561.CrossRefGoogle Scholar
Bomze, D. and Ioannidis, A. , 3D-Printing of High-Strength and Bioresorbable Ceramics for Dental and Maxillofacial Surgery Applications-the LCM Process. Ceramic Applications, 2019. 7(1): p. 38-43.Google Scholar
Ghayor, C. and Weber, F.E. , Osteoconductive Microarchitecture of Bone Substitutes for Bone Regeneration Revisited. Frontiers in physiology, 2018. 9: p. 960-960.CrossRefGoogle ScholarPubMed
Kim, K. , et al., Stereolithographic Bone Scaffold Design Parameters: Osteogenic Differentiation and Signal Expression. Tissue Engineering Part B: Reviews, 2010. 16(5): p. 523-539.CrossRefGoogle ScholarPubMed
Melchels, F.P.W. , et al., Mathematically defined tissue engineering scaffold architectures prepared by stereolithography. Biomaterials, 2010. 31(27): p. 6909-6916.CrossRefGoogle ScholarPubMed
Kebede, M.A. , et al., Stereolithographic and molding fabrications of hydroxyapatite-polymer gels applicable to bone regeneration materials. Journal of the Taiwan Institute of Chemical Engineers, 2018. 92: p. 91-96.CrossRefGoogle Scholar
Dong, P.L. , et al., The Preparation and Characterization of beta-SiAlON Nanostructure Whiskers. Journal of Nanomaterials, 2008.CrossRefGoogle Scholar
Seymour, V.R. and Smith, M.E. , Distinguishing between Structural Models of beta ’-Sialons Using a Combined Solid-State NMR, Powder XRD, and Computational Approach. Journal of Physical Chemistry A, 2019. 123(45): p. 9729-9736.CrossRefGoogle ScholarPubMed
Altun, A. , et al., Dense, Strong, and Precise Silicon Nitride-Based Ceramic Parts by Lithography-Based Ceramic Manufacturing. Applied Sciences, 2020. 10: p. 996.CrossRefGoogle Scholar
Li, Y. , et al., Osteogenic differentiation of mesenchymal stem cells (MSCs) induced by three calcium phosphate ceramic (CaP) powders: A comparative study. Materials Science and Engineering: C, 2017. 80: p. 296-300.CrossRefGoogle ScholarPubMed
Schmidleithner, C. , et al., Application of high resolution DLP stereolithography for fabrication of tricalcium phosphate scaffolds for bone regeneration. Biomedical Materials, 2019. 14(4): p. 045018.CrossRefGoogle ScholarPubMed
Ding, J. , et al., Bone loss and biomechanical reduction of appendicular and axial bones under ketogenic diet in rats. Experimental and therapeutic medicine, 2019. 17(4): p. 2503-2510.Google ScholarPubMed
Wu, X. , et al., Ketogenic Diet Compromises Both Cancellous and Cortical Bone Mass in Mice. Calcified Tissue International, 2017. 101(4): p. 412-421.CrossRefGoogle ScholarPubMed
Bergqvist, A.G.C. , et al., Progressive bone mineral content loss in children with intractable epilepsy treated with the ketogenic diet. The American Journal of Clinical Nutrition, 2008. 88(6): p. 1678-1684.CrossRefGoogle ScholarPubMed
Prabhakar, A. , et al., Acetone as biomarker for ketosis buildup capability--a study in healthy individuals under combined high fat and starvation diets. Nutrition journal, 2015. 14: p. 41-41.CrossRefGoogle Scholar
DIETZ, D.D. , et al., Toxicity Sudies of Acetone Administered in the Drinking Water of Rodents. Toxicological Sciences, 1991. 17(2): p. 347-360.CrossRefGoogle Scholar
Whyte, M.P. , Physiological role of alkaline phosphatase explored in hypophosphatasia. Annals of the New York Academy of Sciences, 2010. 1192(1): p. 190-200.CrossRefGoogle ScholarPubMed
Bonsignore, L.A. , Goldberg, V.M. , and Greenfield, E.M. , Machine oil inhibits the osseointegration of orthopaedic implants by impairing osteoblast attachment and spreading. Journal of Orthopaedic Research, 2015. 33(7): p. 979-987.CrossRefGoogle ScholarPubMed
Faia-Torres, A.B. , et al., Differential regulation of osteogenic differentiation of stem cells on surface roughness gradients. Biomaterials, 2014. 35(33): p. 9023-9032.CrossRefGoogle ScholarPubMed
Faia-Torres, A.B. , et al., Osteogenic differentiation of human mesenchymal stem cells in the absence of osteogenic supplements: A surface-roughness gradient study. Acta Biomaterialia, 2015. 28: p. 64-75.CrossRefGoogle ScholarPubMed
Poon, C.Y. and Bhushan, B. , Comparison of surface roughness measurements by stylus profiler, AFM and non-contact optical profiler. Wear, 1995. 190(1): p. 76-88.CrossRefGoogle Scholar