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Intrafibrillar demineralization study of single human dentin collagen fibrils by AFM

Published online by Cambridge University Press:  17 March 2011

M. Balooch ,
G. Balooch ,
S. Habelitz ,
S. J. Marshall  and
G. W. Marshall
M. Balooch
Affiliation:
Department of Preventive and Restorative Dental Sciences Division of Biomaterials and BioengineeringUniversity of California, San Francisco 707 Parnassus Ave. San Francisco, Ca 94143
G. Balooch
Affiliation:
Department of Preventive and Restorative Dental Sciences Division of Biomaterials and BioengineeringUniversity of California, San Francisco 707 Parnassus Ave. San Francisco, Ca 94143
S. Habelitz
Affiliation:
Department of Preventive and Restorative Dental Sciences Division of Biomaterials and BioengineeringUniversity of California, San Francisco 707 Parnassus Ave. San Francisco, Ca 94143
S. J. Marshall
Affiliation:
Department of Preventive and Restorative Dental Sciences Division of Biomaterials and BioengineeringUniversity of California, San Francisco 707 Parnassus Ave. San Francisco, Ca 94143
G. W. Marshall
Affiliation:
Department of Preventive and Restorative Dental Sciences Division of Biomaterials and BioengineeringUniversity of California, San Francisco 707 Parnassus Ave. San Francisco, Ca 94143
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Abstract

In situ atomic force microscopy (AFM) was used to investigate the kinetics of demineralization of human dentin collagen fibrils. Individual dentin collagen fibrils containing intrafibrillar mineral were isolated, transferred onto a glass slide and exposed to water for a day prior to demineralization studies. The fibrils then were exposed to trypsin for removal of non-collagenous proteins and subsequently demineralized in 10 vol % citric acid. Topographic images showed a gradual increase in gap-overlap depth of the fibril. The gap-overlap depth varied linearly with the square root of time before saturation at 7 nm in approximately sixty minutes, suggesting a diffusion process for demineralization of intrafibrillar mineral. Micro Raman studies of partially demineralized dentin revealed the existence of a phosphate peak at wave number close to 960 cm-1. The peak gradually disappeared in 60 minutes as the samples were exposed to 10% citric acid, supporting the notion that AFM topography may be correlated to the degree of intrafibrillar mineralization.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 823: Symposium W – Biological and Bioinspired Materials and Devices , 2004 , W6.1
Copyright
Copyright © Materials Research Society 2004

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