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Continuous force-displacement relationships for the human red blood cell at different erythrocytic developmental stages of Plasmodium falciparum malaria parasite

Published online by Cambridge University Press:  01 February 2011

John P. Mills ,
Lan Qie ,
Ming Dao ,
Kevin S. W. Tan ,
Chwee Teck Lim  and
Subra Suresh
John P. Mills
Affiliation:
Department of Materials Science and Engineering, Singapore 117576. Republic of Singapore.
Lan Qie
Affiliation:
Division of Bioengineering, Singapore 117576. Republic of Singapore.
Ming Dao
Affiliation:
Department of Materials Science and Engineering, Singapore 117576. Republic of Singapore.
Kevin S. W. Tan
Affiliation:
Department of Microbiology, National University of Singapore, 10 Kent Ridge Crescent, Singapore 117576. Republic of Singapore.
Chwee Teck Lim
Affiliation:
Division of Bioengineering, Singapore 117576. Republic of Singapore.
Subra Suresh
Affiliation:
Department of Materials Science and Engineering, Singapore 117576. Republic of Singapore.
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Abstract

Prior work involving either aspiration of infected cells into micropipette under suction pressure or deformation in laminar shear flow revealed that the malaria parasite Plasmodium (P.) falciparum could result in significant stiffening of infected human red blood cells (RBCs). In this paper, we present optical tweezers studies of progressive changes to nonlinear mechanical response of infected RBCs at different developmental stages of P. falciparum. From early ring stage to late trophozoite and schizont stages, up to an order of magnitude increase in shear modulus was found under controlled mechanical loading by combining experiments with three-dimensional computational simulations. These results provide novel approaches to study changes in mechanical deformability in the advanced stages of parasite development in the erythrocyte, and suggest a significantly greater stiffening of the red blood cell due to P. falciparum invasion than that considered from previous studies.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 844: Symposium Y – Mechanical Properties of Bio-Inspired and Biological Materials , 2004 , Y7.8
Copyright
Copyright © Materials Research Society 2005

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References

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