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Enhanced Human Bone Marrow Mesenchymal Stem Cell Chondrogenic Differentiation on Cold Atmospheric Plasma Modified Cartilage Scaffold

Published online by Cambridge University Press:  19 December 2014

Wei Zhu ,
Michael Keidar  and
Lijie Grace Zhang
Wei Zhu
Affiliation:
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, United States
Michael Keidar
Affiliation:
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, United States
Lijie Grace Zhang
Affiliation:
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, United States Department of Medicine, The George Washington University, Washington, DC, United States
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Abstract

Articular cartilage is prone to degeneration and possesses extremely poor self-healing capacity due to its low cell density and absence of blood vessels. It has extensively reported tissue engineered scaffold can be a promising approach for cartilage repair. However, there still remains an inherent lack of desirable scaffolds that stimulate cartilage regrowth with appropriate functional properties. Therefore, in this study, we develop a biomimetic cartilage substitute comprising of electrospun polycaprolactone (PCL) with cold atmospheric plasma (CAP) modified cell favorable surface and sustained bioactive factor (bovine serum albumin (BSA) or transforming growth factor beta 1 (TGF-β1)) incorporated microspheres inside for improving stem cell chondrogenesis and cartilage regeneration. Scanning electron microscopy (SEM) analysis showed the drug delivery spheres homogeneously distribution in the fibrous scaffold. Furthermore, CAP treatment renders the scaffold’s surface more hydrophilic and results in more specific vitronectin adsorption as illustrated by contact angle and ELISA testing. Our results showed that the CAP treated scaffold can greatly improve growth and chondrogenic differentiation (such as increased glycosaminoglycan (GAG) synthesis) of human bone marrow-derived mesenchymal stem cells (MSCs).

Keywords

biomaterialbiomedicaltissue
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1723: Plasma Processing and Diagnostics for Life Sciences , 2014 , mrsf14-1723-g03-08
Copyright
Copyright © Materials Research Society 2014 

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References

REFERENCES

Adam, C., et al. ., The distribution of cartilage thickness in the knee-joints of old-aged individuals — measurement by A-mode ultrasound. Clinical Biomechanics, 1998. 13(1): p. 1–10.Google ScholarPubMed
Matsiko, A., Levingstone, T.J., and O'Brien, F.J., Advanced Strategies for Articular Cartilage Defect Repair. MATERIALS, 2013. 6(2): p. 637–668.Google ScholarPubMed
Holmes, B., et al. ., Enhanced human bone marrow mesenchymal stem cell functions in novel 3D cartilage scaffolds with hydrogen treated multi-walled carbon nanotubes. Nanotechnology, 2013. 24(36): p. 365102–1-10.Google ScholarPubMed
Fridman, G., et al. ., Applied plasma medicine. Plasma Processes and Polymers, 2008. 5(6): p. 503–533.Google Scholar
Shashurin, A., et al. ., Living tissue under treatment of cold plasma atmospheric jet. Applied Physics Letters, 2008. 75(18): p. 181501–181501-3.Google Scholar
Baldwin, S.P. and Saltzman, W.M., Materials for protein delivery in tissue engineering. Advanced Drug Delivery Reviews, 1998. 33(1-2): p. 71–86.Google Scholar
Ma, Z., et al. ., Cartilage tissue engineering PLLA scaffold with surface immobilized collagen and basic fibroblast growth factor. Biomaterials, 2005. 26(11): p. 1253–1259.Google ScholarPubMed
Wang, M., et al. ., Cold atmospheric plasma for selectively ablating metastatic breast cancer cells. PloS one, 2013. 8(9): p. e73741.Google ScholarPubMed
Li, L., et al. ., The use of hyaluronan to regulate protein adsorption and cell infiltration in nanofibrous scaffolds. Biomaterials, 2012. 33(12): p. 3428–3445.Google ScholarPubMed
Regis, S., et al. ., Fibronectin adsorption on functionalized electrospun polycaprolactone scaffolds: Experimental and molecular dynamics studies. Journal of Biomedical Materials Research - Part A, 2014. 102(6): p. 1697–1706.Google ScholarPubMed
Hicks, B.G., et al. ., Differential affinity of vitronectin versus collagen for synthetic biodegradable scaffolds for urethroplastic applications. Biomaterials, 2011. 32(3): p. 797–807.Google ScholarPubMed
Webster, T.J., et al. ., Mechanisms of enhanced osteoblast adhesion on nanophase alumina involve vitronectin. Tissue engineering, 2001. 7(3): p. 291–301.Google ScholarPubMed
Wang, M., et al. ., Design of biomimetic and bioactive cold plasma-modified nanostructured scaffolds for enhanced osteogenic differentiation of bone marrow-derived mesenchymal stem cells. Tissue Engineering - Part A, 2014. 20(5-6): p. 1060–1071.Google ScholarPubMed