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Lipid Exchange Rates of Conventional and Polymer Stabilized Liposomes

Published online by Cambridge University Press:  01 February 2011

Awad Ahmed ,
Nicole Heldt ,
Gregory Slack  and
Yuzhuo Li
Awad Ahmed
Affiliation:
Department of Chemistry and Center for Advanced Materials Processing, Clarkson University, Potsdam, New York, 13699-5810, USA
Nicole Heldt
Affiliation:
Department of Chemistry and Center for Advanced Materials Processing, Clarkson University, Potsdam, New York, 13699-5810, USA
Gregory Slack
Affiliation:
Department of Chemistry and Center for Advanced Materials Processing, Clarkson University, Potsdam, New York, 13699-5810, USA
Yuzhuo Li
Affiliation:
Department of Chemistry and Center for Advanced Materials Processing, Clarkson University, Potsdam, New York, 13699-5810, USA
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Abstract

Polymer-stabilized liposome systems consisting of polyethylene glycol bound lipids (PEG-lipids) and conventional (nonpolymer stabilized) liposomes were compared in terms of their inter-membrane lipid migration rates. In order to monitor the exchange of lipids between the membranes, 1-hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphocholine (PY-PC), a phospholipid with pyrene attached to the hydrophobic tail, was used to label the liposome. Labeled and unlabeled liposome systems were mixed and fluorescence spectroscopy was used to examine the lipid transfer. More specifically, the relative employed to deduce the exchange kinetics. After labeled and unlabeled liposome systems were mixed, the E/M ratio for PY-PC in a polymer stabilized liposome system decreased by 66% over a period of 80 minutes, while the E/M for PY-PC in a conventional liposome system decreased 70% in less than 2 minutes. This suggests that the exchange rate for lipids in polymer stabilized liposome systems is much slower than that of conventional liposome systems. In addition, the exchange rates for both conventional and polymer stabilized liposome systems are accelerated at an elevated temperature.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 724: Symposium N – Biological and Biomimetic Materials Properties to Function , 2002 , N8.2
Copyright
Copyright © Materials Research Society 2002

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