Skip to main content

Lipid Exchange Rates of Conventional and Polymer Stabilized Liposomes

  • Awad Ahmed (a1), Nicole Heldt (a1), Gregory Slack (a1) and Yuzhuo Li (a1)

Polymer-stabilized liposome systems consisting of polyethylene glycol bound lipids (PEG-lipids) and conventional (nonpolymer stabilized) liposomes were compared in terms of their inter-membrane lipid migration rates. In order to monitor the exchange of lipids between the membranes, 1-hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphocholine (PY-PC), a phospholipid with pyrene attached to the hydrophobic tail, was used to label the liposome. Labeled and unlabeled liposome systems were mixed and fluorescence spectroscopy was used to examine the lipid transfer. More specifically, the relative employed to deduce the exchange kinetics. After labeled and unlabeled liposome systems were mixed, the E/M ratio for PY-PC in a polymer stabilized liposome system decreased by 66% over a period of 80 minutes, while the E/M for PY-PC in a conventional liposome system decreased 70% in less than 2 minutes. This suggests that the exchange rate for lipids in polymer stabilized liposome systems is much slower than that of conventional liposome systems. In addition, the exchange rates for both conventional and polymer stabilized liposome systems are accelerated at an elevated temperature.

Hide All
1. Lasic D.D., Liposomes: form Physics to Applications, Elsevier, New York, 1993.
2. Woodle M.C., Lasic D.D., Biochim. Biophys. Acta., 1113, 171 (1992)
3. Nakajima A., Bull. Chem. Soc. Japan, 44, 3272 (1971)
4. Almagren M., Chem. Phys. Lett., 71, 539 (1980)
5. Doody M.C., Pownall H.J., Kao Y.J., and Smith L.C., Biochemistry, 19, 108 (1980)
6. Sengupta P., Sackmann E., Kuhnle W., and Scholz H.P., Biochim, Biophys. Acta 436, no. 4, 869 (1976)
7. Wolkowicz P.E., Pownall J.H., Pauly D.F., and McMillin-Wood J.B., Biochemistry, 23, 6426 (1984)
8. Ollman M., Schwarzmann G., Sandhoff K., and Galla H., Biochemistry, 26, 5943 (1987)
9. Li W., Xue L., Mayer L.D., and Bally M.B., Biochim. Biophys. Acta., 1513, 193 (2001)
10. Corley P., and Loughrey H.C., Biochim. Biophys. Acta., 1195, 149 (1994)
11. Forster T., Agnew. Chem., 8, 333 (1969)
12. Samerharju P.J., Virtanen J.A., Eklund K.K., Vainio P., and Kinnunen P.K.J, Biochemistry, 24, 2773 (1985)
13. L'Heureux G.P. and Fragata M., J. Photochem. Photobio. B, 3, 53 (1989)
14. Heldt N., Gauger M., Zhao J., Slack G., Pietryka J., Li Y., Reactive and Functional Polymers, 48, 181 (2001)
15. Roseman M.A., and Thompson T.E., Biochemistry, 19, 439 (1980)
16. Galla H., and Hartman W., Chem. Phys. Lipids., 27, 199 (1980)
17. Duportail G., and Lianos P., “Fluorescent probing of vesicles using pyrene and pyrene derivatives derivatives,” in Rosoff M. (Ed.), Vesicles, Marcel Dekker, New York. 1996, pp. 295372.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Online Proceedings Library (OPL)
  • ISSN: -
  • EISSN: 1946-4274
  • URL: /core/journals/mrs-online-proceedings-library-archive
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Full text views

Total number of HTML views: 0
Total number of PDF views: 4 *
Loading metrics...

Abstract views

Total abstract views: 77 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 18th November 2017. This data will be updated every 24 hours.