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Optimization of Mesoporous Silicon Microcavities for Proteomic Sensing

Published online by Cambridge University Press:  01 February 2011

L. A. DeLouise  and
B. L. Miller
L. A. DeLouise
Affiliation:
Department of Dermatology and Center for Future Health, University of Rochester Medical Center, 697 Elmwood Ave, Rochester NY 14642, USA
B. L. Miller
Affiliation:
University of Rochester, Departments of Biochemistry and BioPhysics, University of Rochester Medical Center, 697 Elmwood Ave, Rochester NY 14642, USA
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Abstract

To expand the utility of p+ mesoporous silicon devices in proteomic biosensor applications, we have conducted in-depth studies to quantify the impact of employing a strong base post etch process to modify the microstructure of microcavity sensors tuned to operate in the visible spectrum. Changes in the optical response and quality of the cavity are quantified as a function of potassium hydroxide (KOH) exposure and initial porosity. Our results show that an aqueous ethanol solution containing millimolar concentrations of KOH is an effective means not only to increase the porosity and pore size, thereby enhancing pore infiltration of large (40–50kDa) proteins, but also to fine tune the optical properties of a passive optical devices.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 782: Symposium A – Micro- and Nanosystems , 2003 , A5.3
Copyright
Copyright © Materials Research Society 2004

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References

REFERENCES

1. Chan, S., Horner, S. R., Miller, B. L., Fauchet, P. M. “Identification of Gram Negative Bacteria using Nanoscale Silicon Microcavities”, J. Am. Chem. Soc., 123, 11797–11798 (2001).Google Scholar
2. Chan, S., Fauchet, P. M., Li, Y., Rothberg, L. J., and Miller, B. L., “Porous Silicon Microcavities for Biosensing Applications”, physica status solidi (a), 182, 541 (2000).Google Scholar
3. Collins, B. E., Dancil, K.-P., Abbi, G. and Sailor, M. J., “Determining Protein Size Using an Electrochemically Machined Pore Gradient in Silicon”, Adv. Func. Mat., 12, 187–191 (2002).Google Scholar
4. macBeath, Gavin and Schreiber, Stuart L., “Printing Proteins as Microarrays for High-Throughput Function Determination”, Science, September 8, 1760–1763 (2000).Google Scholar
5. Arwin, H., Gavutis, M., Gustafsson, J., Schultzberg, M., Zangooie, S., Tengvall, P., “Protein adsorption in thin porous silicon layers”, physica status solidi (a), 182, 515–520 (2000).Google Scholar
6. DeLouise, L.A., manuscript in preparation.Google Scholar
7. DeVinney, R., Stein, M., Reinscheid, D., Abe, A., Ruschkowski, S., and Finlay, B. B., “Enterohemorrhagic Escherichia coli O157:H7 Produces Tir, Which Is Translocated to the Host Cell Membrane but Is Not Tyrosine Phosphorylated”, Infection and Immunity, 67(5), 2389–2398, (May 1999).Google Scholar
8. Kenny, B., DeVinney, R., Stein, M., Reinscheid, D. J., Frey, E. A. and Finlay, B. B., “Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells”, Cell, 91, 511–20 (1997).Google Scholar
9. Tinsley-Bown, A.M. et al. Tuning The Pore Size And Surface Chemistry of Porous Silicon For Immunoassays. physica status solidi (a), 182, 547–53 (2000).Google Scholar
10. Hamm, D., Sakka, T., and Ogata, Y. H., “Etching of porous silicon in basic solution”, physica status solidi (a), 197 (1), 175–179 (2003).Google Scholar
11. Steinsland, et.al., Sensors and Actuators A 54, 728–732, 1996 Google Scholar
12. Madou, M., “Fundamentals of Microfabrication”, CRC Press, 1997.Google Scholar
13. Bruggeman, D.A.G., Ann. Phys. 24, 636 (1935).Google Scholar
14. Reece, P.J., Lerondel, , Mulders, G. J., Zheng, W.H., Gal, M., “Fabrication and tuning of high quality porous silicon microcavities”, physica status solidi (a), 197 (2), 321–325 (2003).Google Scholar
15. DeLouise, L.A. et. al., submitted to SPIE proceeding Spring 2004.Google Scholar
16. Color photo pictured on MRS online proceedings, http://www.mrs.org/publications/epubs/proceedings/fall2003/. Google Scholar
17. DeLouise, L.A. et. al., manuscript in progress.Google Scholar
18. Thermal oxidation induces a porosity dependent blue shift. Microcavities fabricated with lower porosity layers exhibit a larger blue shift. The oxidation step also induces a degradation of the microcavity Q factor, which is more severe for samples pretreated in KOH.Google Scholar