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Properties and Applications of Novel DNA-Based Surfactants

Published online by Cambridge University Press:  01 February 2011

Vesselin N. Paunov ,
Chun Xu ,
Pietro Taylor ,
Mustafa Ersoz  and
Paul D.I. Fletcher
Vesselin N. Paunov
Affiliation:
Department of Chemistry, University of Hull, Hull, HU6 7RX, United Kingdom.
Chun Xu
Affiliation:
Department of Chemistry, University of Hull, Hull, HU6 7RX, United Kingdom.
Pietro Taylor
Affiliation:
Department of Chemistry, University of Hull, Hull, HU6 7RX, United Kingdom.
Mustafa Ersoz
Affiliation:
Department of Chemistry, University of Hull, Hull, HU6 7RX, United Kingdom.
Paul D.I. Fletcher
Affiliation:
Department of Chemistry, University of Hull, Hull, HU6 7RX, United Kingdom.
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Abstract

We have designed a novel class of functional surfactants based on DNA and have explored their properties in relation to several possible applications. The DNA-surfactants consist of short chain DNA oligonucleotides covalently bound to a large hydrophobic group, which makes the DNA molecules amphiphilic. We demonstrate that these materials behave like common detergents and are surface-active at various fluid surfaces, e.g. air-water, oil-water interfaces, and lipid bilayers. We also show that once adsorbed the DNA-surfactants remain on the liquid surface upon hybridisation with a complementary DNA chain. We use complementary DNA-surfactants to functionalise fluid surfaces and to program the interactions between them based on Watson-Crick pairing. By selecting the appropriate DNA base sequences, the interaction between the fluid surfaces functionalised with DNA-surfactants can be programmed with a very high specificity. We have also developed a novel procedure for micro-patterning of solid surfaces with DNA by a microcontact printing with aqueous inks of DNA-surfactants which can be utilised for rapid fabrication of DNA assays and genetic biochips.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA6.4
Copyright
Copyright © Materials Research Society 2005

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References

REFERENCES

1. Xu, C., Taylor, P., Ersoz, M., Fletcher, P.D.I., Paunov, V.N., J. Mater. Chem., 10, 3044 (2003).Google Scholar
2. Letsinger, R.L., Alul, R.A., Farooqui, F., Gryaznov, S.M., Kinster, O., Nucleic Acid Res., 24, 75 (1991).Google Scholar
3. Mackellar, C., Grahama, D., Will, D.W., Burgess, S., Brown, T., Nucleic Acid Res., 20, 3411 (1992).Google Scholar
4. Letsinger, R.L., Chaturvedi, S.K., Farooqui, F., Salunke, M., J. Am. Chem. Soc., 115, 305 (1993).Google Scholar
5. Gryaznov, S., Lloyd, D.H., Nucleic Acid Res., 21, 5909 (1993).Google Scholar
6. Manoharan, M., Tivel, K.L., Cook, P.D., Tetrahedron Lett., 36, 3651 (1995).Google Scholar
7. Hotoda, H., Koizumi, M., Koga, R., Momota, K., Ohmine, T., Furukawa, H., Nishigaki, T., Kinoshite, T., Kanoko, M., Nucleosides & Nucleotides, 15, 531 (1996).Google Scholar
8. Tomkins, J.M., Barnes, K.J., Blacker, A.J., Watkins, W.J., Abel, C., Tetrahedron Lett., 38, 691 (1997).Google Scholar
9. Guzaev, A., Lönnberg, H., Tetrahedron, 55, 9101 (1999).Google Scholar
10. Mucic, R.C., Storhoff, J.J., Mirkin, C.A., Lestinger, R.L., J. Am. Chem. Soc., 120, 12674 (1998).Google Scholar
11. Storhoff, J.J., Mirkin, C.A., Chem. Rev., 99, 1849 (1999).Google Scholar
12. Storhoff, J.J., Lazarides, A.A., Mucic, R.C., Mirkin, C.A., Lestinger, R.L., Schatz, G.C., J. Am. Chem. Soc., 122, 4640 (2000).Google Scholar
13. Taton, T.A., Lu, G., Mirkin, C.A., J.|Am. Chem. Soc., 123, 5164 (2001).Google Scholar
14. Xu, C., Taylor, P., Fletcher, P.D.I., Paunov, V.N., J. Mater. Chem., 15, 394, (2005).Google Scholar