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Towards a Tissue Engineered Bladder Wall Patch: The Scaffold

Published online by Cambridge University Press:  15 February 2011

J. G. Hilborn ,
A. Laurent ,
H.-J. Mathieu ,
I. Bisson  and
P. Frey
J. G. Hilborn
Affiliation:
Department of Material Sciences, Swiss Federal Institute of Lausanne, CH-1015 Ecublens, Switzerland, joens.hilborn@epfl.ch
A. Laurent
Affiliation:
Department of Material Sciences, Swiss Federal Institute of Lausanne, CH-1015 Ecublens, Switzerland, joens.hilborn@epfl.ch
H.-J. Mathieu
Affiliation:
Department of Material Sciences, Swiss Federal Institute of Lausanne, CH-1015 Ecublens, Switzerland, joens.hilborn@epfl.ch
I. Bisson
Affiliation:
Pediatric Surgery, Centre Hospitalier du Canton de Vaud, 1011 Lausanne, Switzerland
P. Frey
Affiliation:
Pediatric Surgery, Centre Hospitalier du Canton de Vaud, 1011 Lausanne, Switzerland
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Abstract

A new method for surface functionalization of inherently reactive polymers such as polyesters has been developed. It is based on the direct nucleophilic attack on the ester group in the polyethyleneterephtalate backbone by the acid moiety of polyacrylic acid (PAA) catalyzed by titaniumisopropoxide. The PAA grafted surfaces were characterized by X-ray photoelectron spectroscopy to demonstrated the presence of a thin grafted layer rendering the substrates hydrophilic. Subsequent acid group activation using N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride allowed for in-situ collagen immobilization rendering the surfaces cell adhesive. Human smooth muscle and urothelial cells successfully seeded these surfaces, reaching confluence after 7 days.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 550: Symposium GG/HH/II – Biomedical Materials-Drug Delivery, Implants and Tissue Engr , 1998 , 189
Copyright
Copyright © Materials Research Society 1999

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