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Characterization of Guignardia mangiferae isolated from tropical plants based on morphology, ISSR-PCR amplifications and ITS1-5.8S-ITS2 sequences

Published online by Cambridge University Press:  17 February 2004

Katia F. RODRIGUES ,
Thomas N. SIEBER ,
Christoph R. GRÜNIG  and
Ottmar HOLDENRIEDER
Katia F. RODRIGUES
Affiliation:
FIOCRUZ, Instituto Oswaldo Cruz, Departamento de Micologia, CP 926, BR-21045-900 Rio de Janeiro, RJ, Brazil. E-mail: katia@ioc.fiocruz.br
Thomas N. SIEBER
Affiliation:
Swiss Federal Institute of Technology, Department of Environmental Sciences, Forest Pathology and Dendrology, ETH-Zentrum, CH-8092 Zurich, Switzerland.
Christoph R. GRÜNIG
Affiliation:
Swiss Federal Institute of Technology, Department of Environmental Sciences, Forest Pathology and Dendrology, ETH-Zentrum, CH-8092 Zurich, Switzerland.
Ottmar HOLDENRIEDER
Affiliation:
Swiss Federal Institute of Technology, Department of Environmental Sciences, Forest Pathology and Dendrology, ETH-Zentrum, CH-8092 Zurich, Switzerland.
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Abstract

Phenotypic and genotypic variability of 18 endophytic Guignardia strains from different host plants (Anacardiaceae: Anacardium giganteum, Myracrodruon urundeuva, Spondias mombin; Apocynaceae: Aspidosperma polyneuron; Ericaceae: Rhododendron sp.; Fabaceae: Bowdichia nitida; Leguminosae: Cassia occidentalis; Rutaceae: Citrus aurantium) growing in different sites in Brazil was assessed by means of morphometric measurements and inter-single-sequence-repeat-anchored polymerase chain reaction (ISSR-PCR) amplifications of the DNA. Morphology of conidia and ascospores and growth rates of the Brazilian isolates corresponded well with those of G. mangiferae. Multiple correspondence analysis (MCA) of the ISSR-PCR data yielded three groups of strains, which did, however, not correspond either to the host or to the geographic origin. The same individual tree hosted genotypically different strains indicating multiple infections. Phylogeny based on ITS1-5.8S-ITS2 sequence analyses confirmed conspecificity of the Brazilian isolates with G. mangiferae (syn. G. endophyllicola).

Type
Research Article
Information
Mycological Research , Volume 108 , Issue 1 , January 2004 , pp. 45 - 52
Copyright
The British Mycological Society 2004

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