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NG2-positive glia in the human central nervous system

  • Susan M. Staugaitis (a1) (a2) and Bruce D. Trapp (a1)

Cells that express the NG2 chondroitin sulfate proteoglycan and platelet-derived growth factor receptor alpha (NG2 glia) are widespread in the adult human cerebral cortex and white matter and represent 10–15% of non-neuronal cells. The morphology and distribution of NG2 glia are similar to, but distinct from, both microglia and astrocytes. They are present as early as 17 weeks gestation and persist throughout life. NG2 glia can be detected in a variety of human central nervous system (CNS) diseases, of which multiple sclerosis is the best studied. NG2 glia show morphological changes in the presence of pathology and can show expression of the Ki-67 proliferation antigen. The antigenic profile and morphology of NG2 glia in human tissues are consistent with an oligodendrocyte progenitor function that has been well established in rodent models. Most antibodies to NG2 do not stain formalin-fixed paraffin-embedded tissues. Advances in our understanding of NG2 glia in human tissues will require the development of more robust markers for their detection in routinely processed human specimens.

Corresponding author
Correspondence should be addressed to: Bruce D. Trapp, Department of Neurosciences, Cleveland Clinic, 9500 Euclid Avenue NC30, Cleveland OH 44195, USA phone: 216-444-7177 fax: 216-444-7927 email:
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A. Aguirre and V. Gallo (2004) Postnatal neurogenesis and gliogenesis in the olfactory bulb from NG2-expressing progenitors of the subventricular zone. Journal of Neuroscience 24, 1053010541.

K.L. Baracskay , G.J. Kidd , R.H. Miller and B.D. Trapp (2007) NG2-positive cells generate A2B5-positive oligodendrocyte precursor cells. Glia 55, 10011010.

D.E. Bergles , J.D. Roberts , P. Somogyi and C.E. Jahr (2000) Glutamatergic synapses on oligodendrocyte precursor cells in the hippocampus. Nature 405, 187191.

J. Bu , N. Akhtar and A. Nishiyama (2001) Transient expression of the NG2 proteoglycan by a subpopulation of activated macrophages in an excitotoxic hippocampal lesion. Glia 34, 296310.

J. Bullwinkel , B. Baron-Luhr , A. Ludemann , C. Wohlenberg , J. Gerdes and T. Scholzen (2006) Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells. Journal of Cell Physiology 206, 624635.

T.F. Bumol and R.A. Reisfeld (1982) Unique glycoprotein–proteoglycan complex defined by monoclonal antibody on human melanoma cells. Proceedings of the National Academy of Sciences of the U.S.A. 79, 12451249.

P. Canoll and J.E. Goldman (2008) The interface between glial progenitors and gliomas. Acta Neuropathologica 116, 465477.

A. Chang , W.W. Tourtellotte , R. Rudick and B.D. Trapp (2002) Premyelinating oligodendrocytes in chronic lesions of multiple sclerosis. New England Journal of Medicine 346, 165173.

R. Filipovic , S. Rakic and N. Zecevic (2002) Expression of Golli proteins in adult human brain and multiple sclerosis lesions. Journal of Neuroimmunology 127, 112.

I. Jakovcevski and N. Zecevic (2005) Sequence of oligodendrocyte development in the human fetal telencephalon. Glia 49, 480491.

K.L. Ligon , J.A. Alberta , A.T. Kho , J. Weiss , M.R. Kwaan , C.L. Nutt (2004) The oligodendroglial lineage marker OLIG2 is universally expressed in diffuse gliomas. Journal of Neuropathology and Experimental Neurology 63, 499509.

S.C. Lin and D.E. Bergles (2004) Synaptic signaling between neurons and glia. Glia 47, 290298.

S.C. Lin , J.H. Huck , J.D. Roberts , W.B. Macklin , P. Somogyi and D.E. Bergles (2005) Climbing fiber innervation of NG2-expressing glia in the mammalian cerebellum. Neuron 46, 773785.

Y. Maeda , M. Solanky , J. Menonna , J. Chapin , W. Li and P. Dowling (2001) Platelet-derived growth factor-alpha receptor-positive oligodendroglia are frequent in multiple sclerosis lesions. Annals of Neurology 49, 776785.

R.H. Miller , S. David , R. Patel , E.R. Abney and M.C. Raff (1985) A quantitative immunohistochemical study of macroglial cell development in the rat optic nerve: in vivo evidence for two distinct astrocyte lineages. Developmental Biology 111, 3541.

A.C. Morgan Jr., D.R. Galloway and R.A. Reisfeld (1981) Production and characterization of monoclonal antibody to a melanoma specific glycoprotein. Hybridoma 1, 2736.

A. Nishiyama , A. Chang and B.D. Trapp (1999) NG2+ glial cells: a novel glial cell population in the adult brain. Journal of Neuropathology and Experimental Neurology 58, 11131121.

A. Nishiyama , M. Komitova , R. Suzuki and X. Zhu (2009) Polydendrocytes (NG2 cells): multifunctional cells with lineage plasticity. Nature Reviews. Neuroscience 10, 922.

A. Nishiyama , X.H. Lin and W.B. Stallcup (1995) Generation of truncated forms of the NG2 proteoglycan by cell surface proteolysis. Molecular Biology of the Cell 6, 18191832.

M.C. Nunes , N.S. Roy , H.M. Keyoung , R.R. Goodman , G. McKhann , L. Jiang (2003) Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain. Nature Medicine 9, 439447.

U. Ozerdem , K.A. Grako , K. Dahlin-Huppe , E. Monosov and W.B. Stallcup (2001) NG2 proteoglycan is expressed exclusively by mural cells during vascular morphogenesis. Developmental Dynamics 222, 218227.

G. Pluschke , M. Vanek , A. Evans , T. Dittmar , P. Schmid , P. Itin (1996) Molecular cloning of a human melanoma-associated chondroitin sulfate proteoglycan. Proceedings of the National Academy of Sciences of the U.S.A. 93, 97109715.

S. Pouly , B. Becher , M. Blain and J.P. Antel (1999) Expression of a homologue of rat NG2 on human microglia. Glia 27, 259268.

S. Rakic and N. Zecevic (2003) Early oligodendrocyte progenitor cells in the human fetal telencephalon. Glia 41, 117127.

R. Reynolds , M. Dawson , D. Papadopoulos , A. Polito , I. DiBello , D. Pham-Dinh (2002) The response of NG2-expressing oligodendrocyte progenitors to demyelination in MOG-EAE and MS. Journal of Neurocytology 31, 523536.

L.E. Rivers , K.M. Young , M. Rizzi , F. Jamen , K. Psachoulia , A. Wade (2008) PDGFRA/NG2 glia generate myelinating oligodendrocytes and piriform projection neurons in adult mice. Nature Neuroscience 11, 13921401.

F. Ruffini , N. Arbour , M. Blain , A. Olivier and J.P. Antel (2004) Distinctive properties of human adult brain-derived myelin progenitor cells. American Journal of Pathology 165, 21672175.

N. Sanai , A. Alvarez-Buylla and M.S. Berger (2005) Neural stem cells and the origin of gliomas. The New England Journal of Medicine 353, 811822.

N. Scolding , R. Franklin , S. Stevens , C.-H. Heldin , A. Compston and J. Newcombe (1998) Oligodendrocyte progenitors are present in the normal adult human CNS and in the lesions of multiple sclerosis. Brain 121, 22212228.

Y. Shoshan , A. Nishiyama , A. Chang , S. Mork , G.H. Barnett , J.K. Cowell (1999) Expression of oligodendrocyte progenitor cell antigens by gliomas: implications for the histogenesis of brain tumors. Proceedings of the National Academy of Sciences of the U.S.A. 96, 1036110366.

F.J. Sim , J.K. Lang , B. Waldau , N.S. Roy , T.E. Schwartz , W.H. Pilcher (2006) Complementary patterns of gene expression by human oligodendrocyte progenitors and their environment predict determinants of progenitor maintenance and differentiation. Annals of Neurology 59, 763779.

A.A. Sosunov , X. Wu , H.L. Weiner , C.B. Mikell , R.R. Goodman , P.D. Crino (2008) Tuberous sclerosis: a primary pathology of astrocytes? Epilepsia 49 (Suppl 2), 5362.

H. Takei , S.T. Yogeswaren , K.K. Wong , V. Mehta , M. Chintagumpala , R.C. Dauser (2008) Expression of oligodendroglial differentiation markers in pilocytic astrocytomas identifies two clinical subsets and shows a significant correlation with proliferation index and progression free survival. Journal of Neurooncology 86, 183190.

R. Wigley , N. Hamilton , A. Nishiyama , F. Kirchhoff and A.M. Butt (2007) Morphological and physiological interactions of NG2-glia with astrocytes and neurons. Journal of Anatomy 210, 661670.

H.C. Wilson , N.J. Scolding and C.S. Raine (2006) Co-expression of PDGF alpha receptor and NG2 by oligodendrocyte precursors in human CNS and multiple sclerosis lesions. Journal of Neuroimmunology 176, 162173.

M. Windrem , M.C. Nunes , W.K. Rashbaum , T.H. Schwartz , R.A. Goodman , G. McKhann (2004) Fetal and adult human oligodendrocyte progenitor cell isolates myelinate the congenitally dysmyelinated brain. Nature Medicine 10, 9397.

M.S. Windrem , S.J. Schanz , M. Guo , G.F. Tian , V. Washco , N. Stanwood (2008) Neonatal chimerization with human glial progenitor cells can both remyelinate and rescue the otherwise lethally hypomyelinated shiverer mouse. Cell Stem Cell 2, 553565.

G. Wolswijk (2002) Oligodendrocyte precursor cells in the demyelinated multiple sclerosis spinal cord. Brain 125, 338349.

X. Zhu , D.E. Bergles and A. Nishiyama (2008) NG2 cells generate both oligodendrocytes and gray matter astrocytes. Development 135, 145157.

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Neuron Glia Biology
  • ISSN: 1740-925X
  • EISSN: 1741-0533
  • URL: /core/journals/neuron-glia-biology
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