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Characterization and cross-species transferability of a novel set of microsatellites derived from root transcriptomes of Camellia oleifera

Published online by Cambridge University Press:  21 February 2019

Shuangcheng Wu ,
Hang Ye ,
Yuansong Chen ,
Jiemei Deng ,
Jiexia Su ,
Yayu Xie ,
Qinru Xie ,
Zhaoyuan Zhang ,
Zihai Qin  and
Yufei Xiao
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Shuangcheng Wu
Affiliation:
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University, Qinzhou Guangxi, China
Hang Ye
Affiliation:
Guangxi Key Laboratory of Special Non-wood Forest Cultivation and Utilization, Guangxi Forestry Research Institute, Nanning Guangxi, China
Yuansong Chen
Affiliation:
Guangxi State-owned Sanmenjiang Forestry Farm, Liuzhou Guangxi, China
Jiemei Deng
Affiliation:
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University, Qinzhou Guangxi, China
Jiexia Su
Affiliation:
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University, Qinzhou Guangxi, China
Yayu Xie
Affiliation:
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University, Qinzhou Guangxi, China
Qinru Xie
Affiliation:
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University, Qinzhou Guangxi, China
Zhaoyuan Zhang
Affiliation:
Guangxi Key Laboratory of Special Non-wood Forest Cultivation and Utilization, Guangxi Forestry Research Institute, Nanning Guangxi, China
Zihai Qin
Affiliation:
Guangxi Key Laboratory of Special Non-wood Forest Cultivation and Utilization, Guangxi Forestry Research Institute, Nanning Guangxi, China
Yufei Xiao
Affiliation:
Guangxi Key Laboratory of Special Non-wood Forest Cultivation and Utilization, Guangxi Forestry Research Institute, Nanning Guangxi, China
Xiaoyun Wang*
Affiliation:
Collaborative Innovation Center for the Modern Technology and Industrial Development of Jiangxi Minority Traditional Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang Jiangxi, China
Pengliang Wang*
Affiliation:
Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University, Qinzhou Guangxi, China Guangxi Key Laboratory of Special Non-wood Forest Cultivation and Utilization, Guangxi Forestry Research Institute, Nanning Guangxi, China
*
*Corresponding author. E-mail: pengliang_wang@163.com; wxy20052002@aliyun.com
*Corresponding author. E-mail: pengliang_wang@163.com; wxy20052002@aliyun.com
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Abstract

Camellia oleifera is an important woody plant producing healthy edible oils. People need a large number of molecular markers, especially microsatellite, in breeding of C. oleifera. In this study, we sequenced the root transcriptomes of C. oleifera, and then designed a novel set of microsatellite markers based on the root-expressed genes. We assembled a total of 57,121 unigenes with a length of 42.63 Mb, which harboured 15,902 microsatellites. Among these microsatellites, di-nucleotide repeat motifs were the most abundant group (56.45%), then followed by tri- (25.20%), mono- (12.12%), hexa- (3.21%), penta- (2.18%) and quad-nucleotide ones (0.84%). In total, 6738 primer pairs were designed successfully to amplify the microsatellite loci. To test these microsatellite markers, 48 primer pairs were randomly selected and synthesized and validated in C. oleifera and its eight relatives. Up to 75% of the primer pairs amplified in C. oleifera and its relatives, and 62.5% displayed polymorphism. The transferability and diverse alleles across its eight relatives were detected for each polymorphic primer pair. The novel set of microsatellites derived from the root transcriptomes here provided a useful resource for future molecular genetics improvement of C. oleifera and its relatives.

Keywords

Camellia oleiferamicrosatelliteroot transcriptometransferability
Type
Short Communication
Information
Plant Genetic Resources , Volume 17 , Issue 4 , August 2019 , pp. 371 - 374
Copyright
Copyright © NIAB 2019 

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