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Development and characterization of novel EST-SSR markers for Gentiana straminea Maxim., a traditional Tibetan herb in China and cross-amplification in related species

Published online by Cambridge University Press:  09 May 2024

Tingfeng Cheng ,
Pengcheng Lin ,
Dangwei Zhou Open the ORCID record for Dangwei Zhou [Opens in a new window] ,
Huan Wang ,
Shengbo Shi ,
Jianwei Shen ,
Jing Meng ,
Xing Ye ,
Kun Zheng  and
Xingqiang Hu
...Show all authors
Tingfeng Cheng
Affiliation:
Key Laboratory of Qinghai-Tibetan Plateau Biological Resource Diversity and Protection, State Commission of Nationalities and Religions, Xining 810007, China Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
Pengcheng Lin
Affiliation:
Key Laboratory of Qinghai-Tibetan Plateau Biological Resource Diversity and Protection, State Commission of Nationalities and Religions, Xining 810007, China
Dangwei Zhou*
Affiliation:
Key Laboratory of Qinghai-Tibetan Plateau Biological Resource Diversity and Protection, State Commission of Nationalities and Religions, Xining 810007, China Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
Huan Wang
Affiliation:
Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
Shengbo Shi
Affiliation:
Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
Jianwei Shen
Affiliation:
Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
Jing Meng
Affiliation:
Key Laboratory of Qinghai-Tibetan Plateau Biological Resource Diversity and Protection, State Commission of Nationalities and Religions, Xining 810007, China
Xing Ye
Affiliation:
Key Laboratory of Qinghai-Tibetan Plateau Biological Resource Diversity and Protection, State Commission of Nationalities and Religions, Xining 810007, China
Kun Zheng
Affiliation:
Key Laboratory of Qinghai-Tibetan Plateau Biological Resource Diversity and Protection, State Commission of Nationalities and Religions, Xining 810007, China
Xingqiang Hu
Affiliation:
Key Laboratory of Qinghai-Tibetan Plateau Biological Resource Diversity and Protection, State Commission of Nationalities and Religions, Xining 810007, China
Yuanwen Zhuang
Affiliation:
Key Laboratory of Qinghai-Tibetan Plateau Biological Resource Diversity and Protection, State Commission of Nationalities and Religions, Xining 810007, China
*
Corresponding author: Dangwei Zhou; Email: dangweizhou@sina.com
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Abstract

Gentiana straminea Maxim. (Gentianaceae) is an important traditional Tibetan herb that is mainly distributed on the Qinghai-Tibetan Plateau. Despite its agricultural and pharmacological importance, there remains a paucity of microsatellite markers, particularly expressed sequence tag-simple sequence repeat (EST-SSR) markers, available for this local endemic species. In this study, based on previous Illumina transcriptome data of G. straminea, a total of 96 EST-SSR markers were initially designed and tested. Thirty-two of 96 loci (33.33%) were successfully amplified and verified for validation. Among them, 10 were polymorphic and had clear bands. The polymorphism information content values were 0.09–0.799, the number of alleles per locus ranged from 3 to 14, and the levels of observed and expected heterozygosity were 0.078–0.722 and 0.238–0.884, respectively, which suggested a high level of information. Moreover, cross-amplification was successful for 10 loci in two other related species, Gentiana macrophylla Pallas and Gentiana dahurica Fischer. These EST-SSR markers provide a valuable tool for investigating the genetic diversity related to quantitative traits and population genetic studies on G. straminea and related species in sect. Cruciata Gaudin.

Keywords

cross-speciesEST-SSR markerGentiana straminea Maximpolymorphismsect. Cruciata Gaudin
Type
Short Communication
Information
Plant Genetic Resources , First View , pp. 1 - 6
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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