Hostname: page-component-76fb5796d-skm99 Total loading time: 0 Render date: 2024-04-30T03:55:55.507Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic diversity of mungbean (Vigna radiata L.) germplasm in Indonesia

Published online by Cambridge University Press:  16 July 2014

Puji Lestari ,
Sue Kyung Kim ,
Reflinur ,
Yang Jae Kang ,
Nurwita Dewi  and
Suk-Ha Lee
Puji Lestari
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul151-921, Republic of Korea Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, IAARD, Bogor16111, Indonesia
Sue Kyung Kim
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul151-921, Republic of Korea
Reflinur
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul151-921, Republic of Korea Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, IAARD, Bogor16111, Indonesia
Yang Jae Kang
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul151-921, Republic of Korea
Nurwita Dewi
Affiliation:
Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, IAARD, Bogor16111, Indonesia
Suk-Ha Lee*
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul151-921, Republic of Korea Plant Genomics and Breeding Institute, Seoul National University, Seoul151-921, Republic of Korea
*
* Corresponding author. E-mail: sukhalee@snu.ac.kr
Get access Rights & Permissions [Opens in a new window]

Abstract

Despite widespread mungbean [Vigna radiata (L.) Wilczek] consumption in Indonesia, few molecular studies have been carried out on accessions and available data are minimal. In this study, we used 30 newly developed simple sequence repeat (SSR) markers designed from the mapped sequence scaffolds of the Korean Sunhwanokdu and Gyeonggijaerae 5 mungbean genomes. These markers were used to examine loci in 83 mungbean accessions collected from diverse geographical areas in Indonesia. A total of 107 alleles were detected among the accessions with 29 polymorphic markers. However, the mean of polymorphic information content (0.33) value and diversity index (0.38) value was indicative of low genetic diversity in this germplasm. The mungbean population structure was not clearly differentiated and the number of subpopulations was unclear. Neighbour-joining tree analysis revealed that the genetic cluster did not reflect the geographical origin of the accessions. Interestingly, the most agriculturally improved varieties were genetically similar to some landraces from one of the main mungbean-producing regions. These newly developed SSR markers could be useful for detecting genetic variability as a basis for establishing a conservation strategy for mungbean germplasm with the aim of enhancing Indonesian breeding programmes.

Keywords

genetic diversityIndonesiamungbeanSSRs
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S91 - S94
Copyright
Copyright © NIAB 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Asian Vegetable Research and Development Centre (AVRDC) (2002) High yielding, MYMV-resistant mungbean lines for South Asia. AVRDC Progress Report, Shanhua, Taiwan. AVRDC Publication No. 02-542 . Shanhua: AVRDC, p. 25.Google Scholar
Blair, MW, Diaz, LM, Buendia, HF and Duque, MC (2009) Genetic diversity, seed size associations and population structure of a core collection of common beans (Phaseolus vulgaris L.). Theoretical and Applied Genetics 119: 955–972.Google Scholar
Burle, ML, Fonseca, JR, Kami, JA and Gepts, P (2010) Microsatellite diversity and genetic structure among common bean (Phaseolus vulgaris L.) landraces in Brazil, a secondary center of diversity. Theoretical and Applied Genetics 121: 801–813.Google Scholar
Chankaew, S, Somta, P, Sorajjapinun, W and Srinives, P (2011) Quantitative trait loci mapping of Cercospora leaf spot resistance in mungbean, Vigna radiata (L.) Wilczek. Molecular Breeding 28: 255–264.Google Scholar
Evanno, G, Regnaut, S and Goudet, J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Molecular Ecology 14: 2611–2620.Google Scholar
Falush, D, Stephens, M and Pritchard, JK (2003) Inference of population structure: extension to linked loci and correlated allele frequencies. Genetics 164: 1567–1587.Google Scholar
Felsenstein, J (1985) Confident limit on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.Google Scholar
Gelvin, SB and Schilperoort, RA (1995) Plant Molecular Biology Manual. Norwell, MA: Kluwer Academic Publisher.Google Scholar
Gwag, JG, Dixit, A, Park, YJ, Ma, KH, Kwon, SJ, Cho, GT, Lee, GA, Lee, SK, Kang, HK and Lee, SH (2010) Assessment of genetic diversity and population structure in mungbean. Genes & Genomics 32: 299–308.Google Scholar
Indonesian Agency for Agricultural Research and Development (IAARD) (2012) Kacang hijau di lahan kering (Mungbean in dry land). Sinar Tani edition, 25–31 January, no. 3441: 8–9.Google Scholar
Jambormias, E, Madubun, EL and Hitijanhubessy, FJD (2003) Yield potential, natural genetic variability and qualitative characteristics heritability of local variety Jamdena. Jurnal Pertanian Kepulauan 2: 100–105.Google Scholar
Kumar, SV, Tan, SG, Quah, SC and Yusoff, K (2002) Isolation of microsatellite markers in mungbean, Vigna radiata . Molecular Ecological Notes 2: 96–98.Google Scholar
Lambrides, CJ and Godwin, ID (2007) Mungbean. In: Kole, C (ed.) Genome Mapping and Molecular Breeding in Plants, Vol. 3: Pulses, Sugar and Tuber Crops. Berlin/Heidelberg: Springer, pp. 69–90.Google Scholar
Liu, J (2001) PowerMarker V3.25 Manual. Available at: http://www.powermarker.net.Google Scholar
Liu, K and Muse, SV (2005) PowerMarker: integrated analysis environment for genetic diversity in core collection accessions of wild barley, Hordeum vulgare ssp. Spontaneum . Hereditas 136: 67–73.Google Scholar
Mondini, L, Noorani, A and Pagnotta, MA (2009) Assessing plant genetic diversity by molecular tools. Diversity I: 19–35.Google Scholar
Poehlman, J (1991) The Mungbean. New Delhi: Oxford and IBH Publishing Co, pp. 195–222.Google Scholar
Tripathy, SK, Suchinnata, S, Lenka, D and Sahoo, S (2010) Genetic diversity of mungbean [(Vigna radiata (L.) Wilczek)] genotypes based on SDS-PAGE albumin seed storage protein. Legume Research – An International Journal 33: 54–57.Google Scholar
Van, K, Kang, YJ, Han, K-S, Lee, Y-H, Gwag, J-G, Moon, J-K and Lee, S-H (2013) Genome-wide SNP discovery in mungbean by Illumina HiSeq. Theoretical and Applied Genetics 126: 2017–2027.Google Scholar
Vetriventhan, M, Upadhaya, HD, Anandakumar, CR, Senthilvel, S, Parzies, HK, Bharathi, A, Varshney, RK and Gowda, CLL (2012) Assessing genetic diversity, allelic richness and genetic relationship among races in ICRISAT foxtail millet core collection. Plant Genetic Resources: Characterization and Utilization 10: 214–223.CrossRefGoogle Scholar
Supplementary material: File

Lestari Supplementary Material

Tables S1-S2

Download Lestari Supplementary Material(File)
File 35.1 KB