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Microsatellite high-resolution melting (SSR-HRM) analysis for identification of sweet cherry rootstocks in Greece

  • Ganopoulos Ioannis (a1) (a2), Xanthopoulou Aliki (a1) (a2), Aravanopoulos Filippos (a1) (a3), Kazantzis Konstantinos (a4), Tsaftaris Athanasios (a1) (a2) and Madesis Panagiotis (a1)...

Abstract

Sweet cherries can be grafted onto a wide range of rootstocks belonging to the genus Prunus. The identification of sweet cherry rootstocks using morphological traits is almost impossible particularly during the dormant season, yet it is very important for the grower to know exactly the rootstock as this has a major influence on cultivar performance and agricultural practices. However, DNA-based molecular analysis carried out on actively growing shoot tips, leaves or dormant buds provides a good opportunity to reliably distinguish the rootstocks. Herein, we have integrated high-resolution melting (HRM) analysis, coupled with five simple sequence repeat (SSR) markers, in order to facilitate the identification of the most popular sweet cherry rootstocks in Greece. The five SSR loci used were highly informative and generated a unique melting curve profile of microsatellites for each of the six sweet cherry rootstocks tested. In particular, one marker, BPPCT002, with six HRM profiles was sufficient to discriminate all the sweet cherry rootstocks studied, highlighting its potential use for rootstock identification. Hence, this assay provided a flexible, cost-effective and closed-tube microsatellite genotyping method well suited to sweet cherry rootstock identification.

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Corresponding author

*Corresponding author. E-mail: pmadesis@certh.gr

References

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Chatzicharisis, I and Kazantzis, K (2011) Prunus avium rootstocks. In: Chatzicharisis I (ed.) Monography. Naousa, Greece: Institute of Pomology, pp. 4–27.
Ganopoulos, I, Argiriou, A and Tsaftaris, A (2011) Microsatellite high resolution melting (SSR-HRM) analysis for authenticity testing of protected designation of origin (PDO) sweet cherry products. Food Control 22: 532541.
Reed, GH and Wittwer, CT (2004) Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis. Clinical Chemistry 50: 17481754.
Rom, RC (1982) A new philosophy for peach rootstock development. Fruit Varieties Journal 36: 3436.
Turkoglu, Z, Koc, A, Ercisli, S, Bilgener, S, Akbulut, M, Yildirim, N, Gercekcioglu, R, Esitken, A and Gunes, M (2012) Genetic relationships among Prunus rootstocks for sweet cherry (Prunus avium L.) cultivars. Plant Genetic Resources 1: 17.

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Microsatellite high-resolution melting (SSR-HRM) analysis for identification of sweet cherry rootstocks in Greece

  • Ganopoulos Ioannis (a1) (a2), Xanthopoulou Aliki (a1) (a2), Aravanopoulos Filippos (a1) (a3), Kazantzis Konstantinos (a4), Tsaftaris Athanasios (a1) (a2) and Madesis Panagiotis (a1)...

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