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Microsatellite high-resolution melting (SSR-HRM) analysis for identification of sweet cherry rootstocks in Greece

Published online by Cambridge University Press:  04 September 2013

Ganopoulos Ioannis ,
Xanthopoulou Aliki ,
Aravanopoulos Filippos ,
Kazantzis Konstantinos ,
Tsaftaris Athanasios  and
Madesis Panagiotis
Ganopoulos Ioannis
Affiliation:
Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki570 01, Greece Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki54 124, Greece
Xanthopoulou Aliki
Affiliation:
Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki570 01, Greece Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki54 124, Greece
Aravanopoulos Filippos
Affiliation:
Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki570 01, Greece Laboratory of Forest Genetics and Tree Breeding, Faculty of Forestry and Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, Greece
Kazantzis Konstantinos
Affiliation:
Pomology Institute, Hellenic Agricultural Organisation – ‘DEMETER’, PO Box 122, Naoussa59200, Greece
Tsaftaris Athanasios
Affiliation:
Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki570 01, Greece Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki54 124, Greece
Madesis Panagiotis*
Affiliation:
Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki570 01, Greece
*
*Corresponding author. E-mail: pmadesis@certh.gr
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Abstract

Sweet cherries can be grafted onto a wide range of rootstocks belonging to the genus Prunus. The identification of sweet cherry rootstocks using morphological traits is almost impossible particularly during the dormant season, yet it is very important for the grower to know exactly the rootstock as this has a major influence on cultivar performance and agricultural practices. However, DNA-based molecular analysis carried out on actively growing shoot tips, leaves or dormant buds provides a good opportunity to reliably distinguish the rootstocks. Herein, we have integrated high-resolution melting (HRM) analysis, coupled with five simple sequence repeat (SSR) markers, in order to facilitate the identification of the most popular sweet cherry rootstocks in Greece. The five SSR loci used were highly informative and generated a unique melting curve profile of microsatellites for each of the six sweet cherry rootstocks tested. In particular, one marker, BPPCT002, with six HRM profiles was sufficient to discriminate all the sweet cherry rootstocks studied, highlighting its potential use for rootstock identification. Hence, this assay provided a flexible, cost-effective and closed-tube microsatellite genotyping method well suited to sweet cherry rootstock identification.

Keywords

HRMPrunusrootstocksSSRsweet cherry
Type
Short Communications
Information
Plant Genetic Resources , Volume 12 , Issue 1 , April 2014 , pp. 160 - 163
Copyright
Copyright © NIAB 2013 

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References

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