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Molecular distinction of two closely resembling Morinda species using rbcL and matK loci for quality management of Indian herbal medicines

Published online by Cambridge University Press:  29 November 2012

Rahul R. Nair ,
P. S. Udayan ,
S. Thilaga ,
M. Kavitha ,
R. M. Bharathanandhini ,
A. M. Nizzy  and
D. Ganesh
Rahul R. Nair
Affiliation:
Plant Genetic Improvement Laboratory, Department of Biotechnology, SPK Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412 Tirunelveli District, Tamil Nadu, India
P. S. Udayan
Affiliation:
Department of Botany, Sree Krishna College, Ariyannur 680 102, Thrissur District, Kerala, India
S. Thilaga
Affiliation:
Plant Genetic Improvement Laboratory, Department of Biotechnology, SPK Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412 Tirunelveli District, Tamil Nadu, India
M. Kavitha
Affiliation:
Plant Genetic Improvement Laboratory, Department of Biotechnology, SPK Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412 Tirunelveli District, Tamil Nadu, India
R. M. Bharathanandhini
Affiliation:
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar Madurai 625 021, Tamil Nadu, India
A. M. Nizzy
Affiliation:
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar Madurai 625 021, Tamil Nadu, India
D. Ganesh*
Affiliation:
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar Madurai 625 021, Tamil Nadu, India
*
*Corresponding author. E-mail: ganeshdsneha@yahoo.co.in
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Abstract

Morinda reticulata Gamble and Morinda umbellata Linn. (Rubiaceae) are medicinally important climbers distributed as a mixed population in southern Western Ghats of India. A close morphological resemblance of these two species misleads the harvester in the identification of plant parts for preparation of herbal medicines. Though both species contain anthraquinone derivatives and share common medicinal properties for treating stomach disorders, each of these species has unique curative properties for treating selective diseases. Conventional methods are not reliable for identification of these species due to similarities in morphology. Thus, misidentification often leads to the deterioration of the quality of medicines. Thus, authentication utilizing conserved gene sequences in the chloroplast genome of these two Morinda spp. has been attempted for precise identification. Here we report the use of two barcoding genes (maturase kinase and ribulose 1,5-bisphosphate carboxylase large subunit) to distinguish M. reticulata and M. umbellata based on single nucleotide polymorphism. The present findings can be used for authenticating leaf samples of M. reticulata and M. umbellata.

Keywords

herbal medicinesM. reticulataM. umbellataquality managementRubiaceaesingle nucleotide polymorphism
Type
Short Communication
Information
Plant Genetic Resources , Volume 11 , Issue 1 , April 2013 , pp. 90 - 93
Copyright
Copyright © NIAB 2012

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References

Asahina, H, Shinozaki, J, Masuda, K, Morimitsu, Y and Satake, M (2010) Identification of medicinal Dendrobium species by phylogenetic analyses using matK and rbcL sequences. Journal of Natural Medicine 64: 133–138.CrossRefGoogle ScholarPubMed
Balasubramani, SP and Venkatasubramanian, P (2011) Molecular identification and development of nuclear DNA ITS sequence based marker to distinguish Coscinium fenestratum Gaertn. (Menispermaceae) from its adulterants. Current Trends in Biotechnology and Pharmacy 5: 1163–1172.Google Scholar
Corpet, F (1988) Multiple sequence alignment with hierarchical clustering. Nucleic Acid Research 16: 10881–10890.Google Scholar
Dassanayake MD, Clayton WD (eds) (1998) A Revised Hand Book of the Flora of Ceylon. New Delhi: Oxford IBH Publishing Corporation Private Limited, p. 321.Google Scholar
Gopalan R, Henry AN (2000) Endemic Plants of India. (Camp for the Endemics of Agasthiyamalai Hills, SW Ghats). Dehra Dun: Bishen Singh Mahendra Pal Singh, pp. 307–310.Google Scholar
Ijinu, TP, Anish, N, Shiju, H, George, V and Pushpangadan, P (2011) Home gardens for nutritional and primary health security of rural poor of South Kerala. Indian Journal of Traditional Knowledge 10: 413–428.Google Scholar
Ismail, TS and Sulthana, AP (2008) Standardization of Morinda umbellata Linn. – an effective crude drug for diarrhoea. E-Journal of Chemistry 5: 652–658.Google Scholar
Kaplan, J, Chavan, P, Warude, D and Patwardhan, B (2004) Molecular markers in herbal drug technology. Current Science 87: 159–165.Google Scholar
Kimura, M (1980) A simple method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111–120.CrossRefGoogle ScholarPubMed
Mosihuzzaman, MM and Choudhary, IM (2008) Protocols on safety, efficacy, standardization, and documentation of herbal medicine. Pure and Applied Chemistry 80: 2195–2230.Google Scholar
Nair, LG and Seeni, S (2002) Rapid clonal multiplication of Morinda umbellata Linn. (Rubiaceae), a medicinal liana, through cultures of nodes and shoot tips from mature plant. Phytomorphology 52: 77–81.Google Scholar
Olmstead, RG and Palmer, JD (1994) Chloroplast DNA systematics: a review of methods and data analysis. American Journal of Botany 81: 1205–1224.CrossRefGoogle Scholar
Roonyamarai, W, Rungsihirunrat, K, Vipunngeun, N and Ruangrungsi, N (2011) Microscopic and molecular analyses of selected Morinda species in Thailand. Asian Journal of Traditional Medicines 6: 118–126.Google Scholar
Saitou, N and Nei, M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406–425.Google Scholar
Singh, DR, Srivastava, AK, Srivastava, A and Srivastava, RC (2011) Genetic diversity among the three different Morinda species using RAPD and ISSR markers. Indian Journal of Biotechnology 10: 285–293.Google Scholar
Tamura, K, Peterson, D, Peterson, N, Stecher, G, Nei, M and Kumar, S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28: 2731–2739.Google Scholar
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